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Dry anaerobic ammonia–methane production from chicken manure


The effect of temperature on production of ammonia during dry anaerobic fermentation of chicken manure (CM), inoculated with thermophilic methanogenic sludge, was investigated in a batch condition for 8 days. Incubation temperature did not have a significant effect on the production of ammonia. Almost complete inhibition of production of methane occurred at 55 and 65°C while quite low yields of 8.45 and 6.34 ml g−1 VS (volatile solids) were observed at 35 and 45°C due to a higher accumulation of ammonia. In order to improve the production of methane during dry anaerobic digestion of CM, stripping of ammonia was performed firstly on the CM previously fermented at 65°C for 8 days: the stripping for 1 day at 85°C and pH 10 removed 85.5% of ammonia. The first-batch fermentation of methane for 75 days was conducted next, using the ammonia-stripped CM inoculated with methanogenic sludge at different ratios, (CM: thermophilic sludge) of 1:2, 1:1, and 2:1 on volume per volume basis at both 35 and 55°C. Production of methane improved and was higher than that of the control (without stripping of ammonia) but the yield of 20.4 ml g−1 VS was still low, so second stripping of ammonia was conducted, which resulted in 74.7% removal of ammonia. A great improvement in the production of methane of 103.5 ml g−1 VS was achieved during the second batch for 55 days.

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This work was supported in part by The Iwatani Naoji Foundation’s Research Grant.

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Correspondence to Yutaka Nakashimada.

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Abouelenien, F., Kitamura, Y., Nishio, N. et al. Dry anaerobic ammonia–methane production from chicken manure. Appl Microbiol Biotechnol 82, 757–764 (2009).

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  • Ammonia production
  • Ammonia stripping
  • Chicken manure
  • Dry fermentation
  • Methane fermentation