Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B

  • Sanni P. Voutilainen
  • Harry Boer
  • Marika Alapuranen
  • Janne Jänis
  • Jari Vehmaanperä
  • Anu Koivula
Biotechnologically Relevant Enzymes and Proteins
  • 360 Downloads

Abstract

Two different types of approach were taken to improve the hydrolytic activity towards crystalline cellulose at elevated temperatures of Melanocarpus albomyces Cel7B (Ma Cel7B), a single-module GH-7 family cellobiohydrolase. Structure-guided protein engineering was used to introduce an additional tenth disulphide bridge to the Ma Cel7B catalytic module. In addition, a fusion protein was constructed by linking a cellulose-binding module (CBM) and a linker from the Trichoderma reesei Cel7A to the C terminus of Ma Cel7B. Both approaches proved successful. The disulphide bridge mutation G4C/M70C located near the N terminus, close to the entrance of the active site tunnel of Ma Cel7B, led to improved thermostability (ΔTm = 2.5°C). By adding the earlier found thermostability-increasing mutation S290T (ΔTm = 1.5°C) together with the disulphide bridge mutation, the unfolding temperature was increased by 4°C (mutant G4C/M70C/S290T) compared to that of the wild-type enzyme, thus showing an additive effect on thermostability. Both disulphide mutants had increased activity towards microcrystalline cellulose (Avicel) at 75°C, apparently solely because of their improved thermostability. The addition of a CBM also improved the thermostability (ΔTm = 2.5°C) and caused a clear (sevenfold) increase in the hydrolysis activity of Ma Cel7B towards Avicel at 70°C.

Keywords

Site-directed mutagenesis Cellulase Saccharomyces cerevisiae expression Protein engineering Cellulose 

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Sanni P. Voutilainen
    • 1
  • Harry Boer
    • 1
  • Marika Alapuranen
    • 2
  • Janne Jänis
    • 3
  • Jari Vehmaanperä
    • 2
  • Anu Koivula
    • 1
  1. 1.VTT Technical Research Centre of FinlandEspooFinland
  2. 2.ROAL OyRajamäkiFinland
  3. 3.Department of ChemistryUniversity of JoensuuJoensuuFinland

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