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Synergistic effect of laccase mediators on pentachlorophenol removal by Ganoderma lucidum laccase

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Abstract

Laccases have low redox potentials limiting their environmental and industrial applications. The use of laccase mediators has proven to be an effective approach for overcoming the low redox potentials. However, knowledge about the role played by the mediator cocktails in such a laccase-mediator system (LMS) is scarce. Here, we assembled different dual-agent mediator cocktails containing 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), vanillin, and/or acetovanillone, and compared their mediating capabilities with those of each individual mediator alone in oxidation of pentachlorophenol (PCP) by Ganoderma lucidum laccase. Cocktails containing ABTS and either vanillin or acetovanillone strongly promoted PCP removal compared to the use of each mediator alone. The removal enhancement was correlated with mediator molar ratios of the cocktails and incubation times. Analysis of the kinetic constants for each mediator compound showed that G. lucidum laccase was very prone to react with ABTS rather than vanillin and acetovanillone in the cocktails. Moreover, the presence of the ABTS radical (ABTS+•) and vanillin or acetovanillone significantly enhanced PCP removal concomitant with electron transfer from vanillin or acetovanillone to ABTS+•. These results strongly suggest that vanillin and acetovanillone mediate the reaction between ABTS and PCP via multiple sequential electron transfers among laccase and its mediators.

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Acknowledgment

This research was supported by Korea Ministry of Environment as “The GAIA project” and “The BK21 project”.

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Correspondence to Yoon-Seok Chang.

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Jeon, J., Murugesan, K., Kim, Y. et al. Synergistic effect of laccase mediators on pentachlorophenol removal by Ganoderma lucidum laccase. Appl Microbiol Biotechnol 81, 783–790 (2008). https://doi.org/10.1007/s00253-008-1753-2

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Keywords

  • Laccase-mediator system
  • Ganoderma lucidum laccase
  • Pentachlorophenol