Applied Microbiology and Biotechnology

, Volume 82, Issue 1, pp 49–57 | Cite as

Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM

  • Cuiqing Ma
  • Ailong Wang
  • Jiayang Qin
  • Lixiang Li
  • Xulu Ai
  • Tianyi Jiang
  • Hongzhi Tang
  • Ping Xu
Biotechnological Products and Process Engineering

Abstract

Enhanced 2,3-butanediol (BD) production was carried out by Klebsiella pneumoniae SDM. The nutritional requirements for BD production by K. pneumoniae SDM were optimized statistically in shake flask fermentations. Corn steep liquor powder and (NH4)2HPO4 were identified as the most significant factors by the two-level Plackett–Burman design. Steepest ascent experiments were applied to approach the optimal region of the two factors and a central composite design was employed to determine their optimal levels. The optimal medium was used to perform fed-batch fermentations with K. pneumoniae SDM. BD production was then studied in a 5-l bioreactor applying different fed-batch strategies, including pulse fed batch, constant feed rate fed batch, constant residual glucose concentration fed batch, and exponential fed batch. The maximum BD concentration of 150 g/l at 38 h with a diol productivity of 4.21 g/l h was obtained by the constant residual glucose concentration feeding strategy. To the best of our knowledge, these results were new records on BD fermentation.

Keywords

Klebsiella pneumoniae 2,3-Butanediol Medium optimization Fed batch 

Notes

Acknowledgements

This work was supported by the grants by National Basic Research Program of China (2007CB707803) and Chinese National Programs for High-Technology Research and Development (2006AA02Z244 and 2006AA020101).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Cuiqing Ma
    • 1
  • Ailong Wang
    • 1
  • Jiayang Qin
    • 1
  • Lixiang Li
    • 1
  • Xulu Ai
    • 1
  • Tianyi Jiang
    • 1
  • Hongzhi Tang
    • 2
  • Ping Xu
    • 2
    • 3
  1. 1.State Key Laboratory of Microbial TechnologyShandong UniversityJinanPeople’s Republic of China
  2. 2.Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China
  3. 3.School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China

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