Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM
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Enhanced 2,3-butanediol (BD) production was carried out by Klebsiella pneumoniae SDM. The nutritional requirements for BD production by K. pneumoniae SDM were optimized statistically in shake flask fermentations. Corn steep liquor powder and (NH4)2HPO4 were identified as the most significant factors by the two-level Plackett–Burman design. Steepest ascent experiments were applied to approach the optimal region of the two factors and a central composite design was employed to determine their optimal levels. The optimal medium was used to perform fed-batch fermentations with K. pneumoniae SDM. BD production was then studied in a 5-l bioreactor applying different fed-batch strategies, including pulse fed batch, constant feed rate fed batch, constant residual glucose concentration fed batch, and exponential fed batch. The maximum BD concentration of 150 g/l at 38 h with a diol productivity of 4.21 g/l h was obtained by the constant residual glucose concentration feeding strategy. To the best of our knowledge, these results were new records on BD fermentation.
KeywordsKlebsiella pneumoniae 2,3-Butanediol Medium optimization Fed batch
This work was supported by the grants by National Basic Research Program of China (2007CB707803) and Chinese National Programs for High-Technology Research and Development (2006AA02Z244 and 2006AA020101).
- Berbert-Molina MA, Sato S, Silveira MM (2001) Ammonium phosphate as a sole nutritional supplement for the fermentative production of 2,3-butanediol from sugar cane juice. Z Naturforsch 56:787–791Google Scholar
- Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore, pp 181–235Google Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
- Syu MJ, Tsao GT, Choudhury T, Cooks RG (1993) Neural network modeling of butanediol fermentation monitored with tandem mass-spectrometry. Abstr Pap Am Chem Soc 205:1–BTECGoogle Scholar
- Yu EKC, Saddler JN (1983) Fed-batch approach to production of 2,3-butanediol by Klebsiella pneumoniae grown on high substrate concentrations. Appl Environ Microbiol 46:630–635Google Scholar
- Zeng AP, Biebl H, Deckwer WD (1990) Effect of pH and acetic acid on the growth and 2,3-butanediol production of Enterobacter aerogenes in continuous culture. Appl Microbiol Biotechnol 33:485–489Google Scholar