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Hyper production of cellulase-free xylanase by Thermomyces lanuginosus SSBP on bagasse pulp and its application in biobleaching

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Abstract

A cellulase-free xylanase production by Thermomyces lanuginosus SSBP using bagasse pulp was examined under submerged (SmC) and solid-state cultivation (SSC). Higher level of xylanase activity (19,320 ± 37 U g−1 dried carbon source) was obtained in SSC cultures than in SmC (1,772 ± 15 U g−1 dried carbon source) after 120 h with 10% inoculum. The biobleaching efficacy of crude xylanase was tested on bagasse pulp, and the maximum brightness of 46.1 ± 0.06% was observed with 50 U of crude xylanase per gram of pulp, which was 3.8 points higher than the brightness of untreated samples. Reducing sugars (26 ± 0.1 mg g−1) and UV-absorbing lignin-derived compounds in the pulp filtrates were observed as maximum in 50 U of crude xylanase-treated samples. T. lanuginosus SSBP has potential applications due to its high productivity of xylanase and its efficiency in pulp bleaching.

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Acknowledgments

This study was supported by grants from the National Research Foundation, Republic of South Africa. The authors are thankful to Prof. Peter Biely, Slovak Academy of Sciences, Bratislava, Slovakia, and Prof. Bernard Prior, University of Stellenbosch, South Africa, for their valuable suggestions, inputs, and critical evaluation of the manuscript. The authors are thankful to Mr. Ray Burger and Mr. Teddy (Sappi Fine Paper, Stanger, South Africa) for their technical assistance.

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Correspondence to A. Manimaran.

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Manimaran, A., Kumar, K.S., Permaul, K. et al. Hyper production of cellulase-free xylanase by Thermomyces lanuginosus SSBP on bagasse pulp and its application in biobleaching. Appl Microbiol Biotechnol 81, 887–893 (2009). https://doi.org/10.1007/s00253-008-1693-x

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Keywords

  • Thermomyces lanuginosus
  • Solid-state cultivation
  • Submerged cultivation
  • Bagasse pulp
  • Biobleaching
  • Brightness
  • Kappa number
  • Dried carbon source