Applied Microbiology and Biotechnology

, Volume 81, Issue 4, pp 629–636 | Cite as

Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans

  • Yanqun Li
  • Mark Horsman
  • Bei Wang
  • Nan Wu
  • Christopher Q. Lan
Biotechnological Products and Process Engineering

Abstract

Microalgal lipids are the oils of future for sustainable biodiesel production. However, relatively high production costs due to low lipid productivity have been one of the major obstacles impeding their commercial production. We studied the effects of nitrogen sources and their concentrations on cell growth and lipid accumulation of Neochloris oleoabundans, one of the most promising oil-rich microalgal species. While the highest lipid cell content of 0.40 g/g was obtained at the lowest sodium nitrate concentration (3 mM), a remarkable lipid productivity of 0.133 g l−1 day−1 was achieved at 5 mM with a lipid cell content of 0.34 g/g and a biomass productivity of 0.40 g l−1 day−1. The highest biomass productivity was obtained at 10 mM sodium nitrate, with a biomass concentration of 3.2 g/l and a biomass productivity of 0.63 g l−1 day−1. It was observed that cell growth continued after the exhaustion of external nitrogen pool, hypothetically supported by the consumption of intracellular nitrogen pools such as chlorophyll molecules. The relationship among nitrate depletion, cell growth, lipid cell content, and cell chlorophyll content are discussed.

Keywords

Lipid Microalgal oils Microalgae Biofuel Biodiesel Nitrogen-starvation 

References

  1. Barnhart MC (2006) Buckets of muckets: a compact system for rearing juvenile freshwater mussels. Aquaculture 254(1–4):227–233CrossRefGoogle Scholar
  2. Becker EW (1994) Microalgae: biotechnology and microbiology. Cambridge University Press, New YorkGoogle Scholar
  3. Bigogno C, Khozin-Goldberg I, Boussiba S, Vonshak A, Cohen Z (2002) Lipid and fatty acid composition of the green oleaginous alga Parietochloris incisa, the richest plant source of arachidonic acid. Phytochemistry 60(5):497–503CrossRefGoogle Scholar
  4. Cheng-Wu Z, Cohen Z, Khozin-Goldberg I, Richmond A (2002) Characterization of growth and arachidonic acid production of Parietochloris incisa comb. nov (Trebouxiophyceae, Chlorophyta). J Appl Phycol 14:453–460CrossRefGoogle Scholar
  5. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306CrossRefGoogle Scholar
  6. Gatenby CM, Orcutt DM, Kreeger DA, Parker BC, Jones VA, Neves RJ (2003) Biochemical composition of three algal species proposed as food for captive freshwater mussels. J Appl Phycol 15:1–11CrossRefGoogle Scholar
  7. Gatenby CM, Parker BC, Neves RJ (1997) Growth and survival of juvenile rainbow mussels, Villosa iris (Lea, 1829) (Bivalvia: Unionidae), reared on algal diets and sediment. Am Malacol Bull 14:57–66Google Scholar
  8. Gordillo FJL, Goutx M, Figueroa FL, Niell FX (1998) Effects of light intensity, CO2 and nitrogen supply on lipid class composition of Dunaliella viridis. J Appl Phycol 10:135–144CrossRefGoogle Scholar
  9. Illman AM, Scragg AH, Shales SW (2000) Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enz Microb Technol 27:631–635CrossRefGoogle Scholar
  10. Jones JW, Neves RJ (2002) Life history and propagation of the endangered fanshell pearly mussel, Cyprogenia stegaria Rafinesque (Bivalvia: Unionidae). J North Am Benthol Soc 21:76–88CrossRefGoogle Scholar
  11. Jones JW, Mair RA, Neves RJ (2005) Factors affecting survival and growth of juvenile freshwater mussels cultured in recirculating aquaculture systems. North Am J Aquacult 67:210–220CrossRefGoogle Scholar
  12. Jovanovic M, Djukic M, Vasiljevic I, Ninkovic M, Jovanovic M (2007) Determination of nitrate by the IE-HPLC-UV method in the brain tissues of Wistar rats poisoned with paraquat. J Serb Chem Soc 72:347–356CrossRefGoogle Scholar
  13. Khozin-Goldberg I, Cohen Z (2006) The effect of phosphate starvation on the lipid and fatty acid composition of the fresh water eustigmatophyte Monodus subterraneus. Phytochem 67:696–701CrossRefGoogle Scholar
  14. Khozin-Goldberg I, Bigogno C, Shrestha P, Cohen Z (2002) Nitrogen starvation induces the accumulation of arachidonic acid in the freshwater green alga Parietochloris incisa (Trebuxiophyceae). J Phycol 38:991–994CrossRefGoogle Scholar
  15. Khozin-Goldberg I, Shrestha P, Cohen Z (2005) Mobilization of arachidonyl moieties from triacylglycerols into chloroplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris incisa. Biochim Biophys Acta Mol Cell Biol Lipids 1738:63–71CrossRefGoogle Scholar
  16. Li Y, Qin JG (2005) Comparison of growth and lipid content in three Botryococcus braunii strains. J Appl Phycol 17:551–556CrossRefGoogle Scholar
  17. Li Y, Horsman M, Wu N, Lan CQ, Dubois-Calero N (2008) Biofuels from microalgae. Biotechnol Prog (in press) doi:10.1021/bp070371kS8756-7938(07)00371-2
  18. Liu ZY, Wang GC, Zhou BC (2008) Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresour Technol 99(11):4717–4722CrossRefGoogle Scholar
  19. Merzlyak MN, Chivkunova OB, Gorelova OA, Reshetnikova IV, Solovchenko AE, Khozin-Goldberg I, Cohen Z (2007) Effect of nitrogen starvation on optical properties, pigments, and arachidonic acid content of the unicellular green alga Parietochloris incisa (Trebouxiophyceae, Chlorophyta). J Phycol 43:833–843CrossRefGoogle Scholar
  20. Metzger P, Largeau C (2005) Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Appl Microbiol Biotechnol 66:486–496CrossRefGoogle Scholar
  21. Solovchenko AE, Khozin-Goldberg I, Didi-Cohen S, Cohen Z, Merzlyak MN (2008) Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa. J Appl Phycol 20:245–251CrossRefGoogle Scholar
  22. Sugimoto K, Midorikawa T, Tsuzuki M, Sato N (2008) Upregulation of PG synthesis on sulfur-starvation for PS I in Chlamydomonas. Biochem Biophys Res Commun 369:660–665CrossRefGoogle Scholar
  23. Takagi M, Watanabe K, Yamaberi K, Yoshida T (2000) Limited feeding of potassium nitrate for intracellular lipid and triglyceride accumulation of Nannochloris sp. UTEX LB1999. Appl Microbiol Biotechnol 54:112–117CrossRefGoogle Scholar
  24. Takagi M, Karseno S, Yoshida T (2006) Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells. J Biosci Bioeng 101:223–226CrossRefGoogle Scholar
  25. Tornabene TG, Holzer G, Lien S, Burris N (1983) Lipid composition of the nitrogen starved green alga Neochloris oleoabundans. Enzyme Microb Technol 5:435–440CrossRefGoogle Scholar
  26. Usui N, Ikenouchi M (1997) The biological CO2 fixation and utilization project by RITE(1): Highly-effective photobioreactor system. Energy Convers Manag 38(suppl 1):S487–S492CrossRefGoogle Scholar
  27. Xiong W, Li X, Xiang J, Wu Q (2008) High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Appl Microbiol Biotechnol 78:29–36CrossRefGoogle Scholar
  28. Xu H, Miao X, Wu Q (2006) High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. J Biotechnol 126:499–507CrossRefGoogle Scholar
  29. Yamaberi K, Takagi M, Yoshida T (1998) Nitrogen depletion for intracellular triglyceride accumulation to enhance liquefaction yield of marine microalgal cells into a fuel oil. J Mar Biotechnol 6:44–48Google Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Yanqun Li
    • 1
    • 2
  • Mark Horsman
    • 1
  • Bei Wang
    • 1
  • Nan Wu
    • 1
  • Christopher Q. Lan
    • 1
  1. 1.Department of Chemical EngineeringUniversity of OttawaOttawaCanada
  2. 2.College of Life ScienceSouth China Normal UniversityGuangzhouChina

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