At present, more than 100 strains of Lentinula edodes are cultivated on a commercial scale in China. A simple, reliable, and effective method to distinguish some commercial strains of the superior type from other commercial strains is very important for the Lentinula industry. In this study, 23 commercial strains of L. edodes cultivated widely in China at present were collected and analyzed with randomly amplified polymorphic DNA (RAPD) technique. Three informative dominant sequence characterized amplified region (SCAR) markers were developed by designing three pairs of specific SCAR primers from three sequenced differential RAPD bands, respectively. Based on the three SCAR markers, three different multiplex polymerase chain reaction (PCR) phenotypes were detected among the 23 studied commercial strains and in which a multilocus phenotype characterizing a commercial strain Cr02 of the superior type could potentially be used to distinguish this strain from the other 22 studied commercial strains. To our knowledge, this study is the first to describe the development of a multiplex PCR technique based on SCAR markers for detecting the molecular phenotypes among commercial strains of L. edodes in China.
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This work was supported by the Natural Science Foundation of China (Grant No. 30700635), the Natural Science Foundation of Zhejiang Province of China (Grant No. Y304403), the Key Project of Science and Technology Programme of Zhejiang Science Technology Department, China (Grant Nos. 2006F11003 and 2005C22057) and the Training Program for Young Scientists of Zhejiang Province, China (Grant No. 2006R20006).
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Li, H., Wu, X., Peng, H. et al. New available SCAR markers: potentially useful in distinguishing a commercial strain of the superior type from other strains of Lentinula edodes in China. Appl Microbiol Biotechnol 81, 303–309 (2008). https://doi.org/10.1007/s00253-008-1671-3
- Lentinula edodes
- Commercial strain
- Superior type
- SCAR marker
- Multiplex PCR
- Molecular phenotype