Optimization of the production of rhamnolipids by Pseudomonas aeruginosa UFPEDA 614 in solid-state culture

  • Doumit Camilios Neto
  • Joel Alexandre Meira
  • Janete Magali de Araújo
  • David Alexander Mitchell
  • Nadia KriegerEmail author
Biotechnological Products and Process Engineering


In recent years, biosurfactants have attracted attention because of their low toxicity, high biodegradability, and good ecological acceptability. However, their production in submerged liquid culture is hampered by the severe foaming that occurs. Solid-state cultivation can avoid this problem. In the current work, we optimized the production of a rhamnolipid biosurfactant by Pseudomonas aeruginosa UFPEDA 614, grown on a solid medium impregnated with a solution containing glycerol. During the study, we increased the production of the biosurfactant over tenfold, with levels reaching 172 g of rhamnolipid per kilogram of dry initial substrate after 12 days. On the basis of the volume of impregnating solution added to the solid support, this yield is of the order of 46 g/L, which is comparable with the best results that have been obtained to date in submerged liquid cultivation. Our results suggest that there is a great potential for using solid-state cultivation for the production of rhamnolipids.


Rhamnolipids Biosurfactants Solid-state cultivation Solid-state fermentation Pseudomonas aeruginosa 



The work was supported financially by Corn Products Brasil and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil), a Brazilian government agency for the advancement of science and technology. Research scholarships were granted to David Mitchell and Nadia Krieger by CNPq and to Doumit Camilios Neto by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil).


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Doumit Camilios Neto
    • 1
  • Joel Alexandre Meira
    • 2
  • Janete Magali de Araújo
    • 3
  • David Alexander Mitchell
    • 1
  • Nadia Krieger
    • 2
    • 4
    Email author
  1. 1.Departamento de Bioquímica e Biologia MolecularUniversidade Federal do ParanáCuritibaBrazil
  2. 2.Departamento de QuímicaUniversidade Federal do ParanáCuritibaBrazil
  3. 3.Departamento de AntibióticosUniversidade Federal de PernambucoRecifeBrazil
  4. 4.Department of ChemistryFederal University of ParanáCuritibaBrazil

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