To reduce the pungency of Capsicum without the loss of its biological activity, a Capsicum sp. was fermented by Bacillus subtilis with the addition of Rapidase enzyme. At 1 day of fermentation, the capsaicin content of the Capsicum ferment with Rapidase had sharply decreased from an initial content of 11.8 to 2.8 μg/ml. The Rapidase-fermented Capsicum had higher total flavonoid and phenolic contents than the Capsicum ferment without Rapidase. In addition, ABTS radical scavenging activity was enhanced in the Rapidase-fermented Capsicum as compared to that without Rapidase. Overall, fermentation using B. subtilis and Rapidase was an efficient method to produce a non-pungent Capsicum with antioxidant properties.
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Arnous A, Makris DP, Kefalas P (2002) Anthocyanin composition and colour characteristics of selected aged wines produced in Greece. J Wine Res 13:23–34
Brummell DA, Harpster MH (2001) Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol Biol 47:311–340
Choi YM, Suh HJ (2004) Pharmacological effects of fermented red pepper. Phytother Res 18:884–888
Cordell GA, Araiyo OE (1993) Capsaicin identification, nomenclature and pharmacotherapy. Ann Pharmacother 27:330–336
DSM (2001) Food specialties, rapidase carrot juice, product sheet. DMS, Delft, The Netherlands
Galati G, Sabzevari O, Wilson JX, O’Brien PJ (2002) Prooxidant activity and cellular effects of the phenoxyl radicals of dietary flavonoids and other polyphenolics. Toxicol 177:91–104
Hanasaki Y, Ogawa S, Fukui S (1994) The correlation between active oxygen scavenging and antioxidative effects of flavonoids. Free Radic Biol Med 16:845–850
Miller GE (1959) Use of DNS a reagent for determination of reducing sugars. Anal Chem 31:426–428
Ohnuki K, Haramizu S, Oki K, Watanabe T, Yazawa S, Fushiki T (2001a) Administration of capsiate, a non-pungent capsaicin analog, promotes energy metabolism and suppress body fat accumulation in mice. Biosci Biotechnol Biochem 65:2735–2740
Ohnuki K, Niwa S, Maeda S, Inoue N, Yazawa S, Fushiki T (2001b) CH-19 Sweet, a non-pungent cultivar of red pepper, increased body temperature and oxygen consumption in humans. Biosci Biotechnol Biochem 65:2200–2204
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237
Schweiggert U, Carle R, Schieber A (2006) Characterization of major and minor capsaicinoids and related compounds in chili pods (Capsicum frutescens L.) by high-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry. Anal Chim Acta 557:236–244
Surh YJ (2002) Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Food Chem Toxicol 40:1091–1097
Topuz A, Ozdemir F (2004) Influences of gamma irradiation and storage on the capsaicinoids of sun-dried and dehydrated paprika. Food Chem 86:509–515
Wakabayashi K, Hoson T, Huber DJ (2003) Methyl de-esterification as a major factor regulating the extent of pectin depolymerization during fruit ripening: a comparison of the action of avocado (Persea americana) and tomato (Lycopersicon esculentum) polygalacturonases. J Plant Physiol 160:667–673
Yazawa S, Suetome N, Okamoto K, Namiki T (1989) Content of capsaicinoids and capsaicinoid-like substances in fruit of pepper (Capsicum annuum L.) hybrids made with “CH-19 Sweet” as a parent. Jpn Soc Hortic Sci 58:601–607
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Lee, S., Kim, S., Lee, J. et al. Nonpungent Capsicum fermentation by Bacillus subtilis and the addition of Rapidase. Appl Microbiol Biotechnol 81, 257–262 (2008). https://doi.org/10.1007/s00253-008-1658-0
- ABTS radical scavenging activity
- Bacillus subtilis
- Capsicum fermentation