Pleurotus ostreatus showed atypical laccase production in submerged vs. solid-state fermentation. Cultures grown in submerged fermentation produced laccase at 13,000 U l−1, with a biomass production of 5.6 g l−1 and four laccase isoforms. However, cultures grown in solid-state fermentation had a much lower laccase activity of 2,430 U l−1, biomass production of 4.5 g l−1, and three laccase isoforms. These results show that P. ostreatus performs much better in submerged fermentation than in solid-state fermentation. This is the first report that shows such atypical behavior in the production of extracellular laccases by fungi.
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Acuña-Argüelles ME, Gutiérrez-Rojas M, Viniegra-González G, Favela-Torres E (1995) Production and properties of three pectinolytic activities produced by Aspergillus niger in submerged and solid-state fermentation. Appl Microbiol Biotechnol 43:808–814
Aguilar CN, Augur C, Favela-Torres E, Viniegra-González G (2001) Production of tannase by Aspergillus niger Aa-20 in submerged and solid-state fermentation: influence of glucose and tannic acid. J Ind Microbiol Biotechnol 26:296–302
Antier P, Minjares A, Roussos S, Raimbault M, Viniegra G (1993) Pectinase-hyperproducing mutants of Aspergillus niger C28B25 for solid-state fermentation of coffee pulp. Enzyme Microb Technol 15:254–260
Ashokkumar B, Gunasekaran P (2002) Beta-fructofuranosidase production by 2-deoxyglucose resistant mutants of Aspergillus niger in submerged and solid-state fermentation. Indian J Exp Biol 40:1032–1037
Asther M, Haon M, Roussos S, Record E, Delattre M, Lesage-Meessen L, Labat M, Asther M (2002) Feruloyl esterase from Aspergillus niger: a comparison of the production in solid state and submerged fermentation. Process Biochem 38:685–691
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Díaz-Godínez G, Soriano J, Augur C, Viniegra-González G (2001) Exopectinases produced by Aspergillus niger in solid-state and submerged fermentation: a comparative study. J Ind Microbiol Biotechnol 26:271–275
Hölker U, Höfer M, Lenz J (2004) Biotechnological advantages of laboratory-scale solid-state fermentation with fungi. Appl Microbiol Biotechnol 64:175–186
Kunitz M (1947) Crystalline soy bean trypsin inhibitor. General properties. J Gen Physiol 30:291
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Maldonado MC, Strasser de Saad AM (1998) Production of pectinesterase and polygalacturonase by Aspergillus niger in submerged and solid state systems. J Ind Microbiol Biotechnol 20:34–38
Palmieri G, Cennamo G, Faraco V, Amoresano A, Sannia G, Giardina P (2003) Atypical laccase isoenzymes from copper supplemented Pleurotus ostreatus cultures. Enzyme Microb Technol 33:220–230
Ramírez NE, Vargas MC, Ariza JC, Martínez C (2003) Caracterización de la lacasa obtenida por dos métodos de producción con Pleurotus ostreatus. Rev Colombiana Biotecnol 2:64–72
Romero-Gómez S, Augur C, Viniegra-González G (2000) Invertase production by Aspergillus niger in submerged and solid-state fermentation. Biotechnol Lett 22:1255–1258
Téllez-Jurado A, Arana-Cuenca A, González-Becerra AE, Viniegra-González G, Loera O (2005) Expression of a heterologous laccase by Aspergillus niger cultured by solid-state and submerged fermentations. Enzyme Microb Technol 38:665–669
Téllez-Téllez M, Sánchez C, Loera O, Díaz-Godínez G (2005) Differential patterns of constitutive intracellular laccases of the vegetative phase for Pleurotus species. Biotechnol Lett 27:1391–1394
Temp U, Eggert C (1999) Novel interaction between laccase and cellobiose dehydrogenase during pigment synthesis in the white rot fungus. Appl Environ Microbiol 65(5):389–395
Tlecuitl-Beristain S, Sánchez C, Loera O, Robson GD, Díaz-Godínez G (2008) Laccases of Pleurotus ostreatus observed at different phases of its growth in submerged fermentation: Production of a novel laccase isoform. Myc Res. doi:https://doi.org/10.1016/j.mycres.2008.03.001
Viniegra-González G, Favela-Torres E, Aguilar CN, Rómero-Gómez SJ, Díaz-Godínez G, Augur C (2003) Advantages of fungal enzyme production in solid state over liquid fermentation systems. Biochem Eng J 13:157–167
Zhu Y, Knol W, Smits JP, Bol J (1996) Medium optimization for nuclease P1 production by Penicillium citrinum in solid-state fermentation using polyurethane foam as inert carrier. Enzyme Microb Technol 18:108–112
We thank Dr. A. L. Demain for critical reading of the manuscript and the Mexican Council of Science and Technology (CONACYT) for supporting this research (Project No. 47396). M. Téllez-Téllez was supported by a CONACYT scholarship (No. 177867).
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Téllez-Téllez, M., Fernández, F.J., Montiel-González, A.M. et al. Growth and laccase production by Pleurotus ostreatus in submerged and solid-state fermentation. Appl Microbiol Biotechnol 81, 675–679 (2008). https://doi.org/10.1007/s00253-008-1628-6