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Laccase-induced C–N coupling of substituted p-hydroquinones with p-aminobenzoic acid in comparison with known chemical routes

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Abstract

Fungal laccases (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) from Pycnoporus cinnabarinus and Myceliophthora thermophila were used as biocatalysts for enzymatic reaction of halogen-, alkyl-, alkoxy-, and carbonyl-substituted p-hydroquinones (laccase substrates) with p-aminobenzoic acid (no laccase substrate). During this reaction, the laccase substrate was oxidized to the corresponding quinones, which react with p-aminobenzoic acid by amination of the laccase substrate. The different substitutions at the hydroquinone substrates were used to prove whether the substituents influence the position of amination and product yields. The cross-coupling of methoxy-p-hydroquinone (alkoxylated) and 2,5-dihydroxybenzaldehyd (carbonyl-substituted) with p-aminobenzoic acid resulted in the formation of one monoaminated product (yield alkoxylated 52%). If monohalogen- or monoalkyl-substituted p-hydroquinones were used as laccase substrates, two monoaminated products (constitution isomers) were formed. The simultaneous formation of two different monoaminated products from the same hydroquinone substrate is the first report for laccase-mediated synthesis of aminated constitution isomers. Depending from the type of substituent of the hydroquinone, the positions of the two monoaminations are different. While the amination at the monoalkylated hydroquinone occurs at the 5- and 6-positions (yield 38%), the amination at monohalogenated hydroquinones was detectable at the 3- and 5-positions (yield 53%). The same product pattern could be achieved if instead of the biocatalyst laccase the chemical catalyst sodium iodate was used as the oxidant. However, the yields were partially much lower (0–45% of the yields with laccase).

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Acknowledgment

We thank K. Weisz (Institute of Biochemistry, University of Greifswald) for providing NMR data and R. Jack (Institute of Immunology, University of Greifswald) for help in preparing the manuscript.

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Correspondence to Annett Mikolasch.

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Laccase-induced C–N coupling of substituted p-hydroquinones with p-aminobenzoic acid in comparison with known chemical routes (DOC 779 KB)

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Mikolasch, A., Matthies, A., Lalk, M. et al. Laccase-induced C–N coupling of substituted p-hydroquinones with p-aminobenzoic acid in comparison with known chemical routes. Appl Microbiol Biotechnol 80, 389 (2008). https://doi.org/10.1007/s00253-008-1595-y

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Keywords

  • Laccase
  • Coupling
  • Hydroquinones
  • p-aminobenzoic acid