Expression of ethylene-forming enzyme (EFE) of Pseudomonas syringae pv. glycinea in Trichoderma viride

  • Li Tao
  • Hong-Jun Dong
  • Xi Chen
  • San-Feng Chen
  • Tian-Hong Wang
Biotechnological Products and Process Engineering

Abstract

The efe gene encoding an ethylene-forming enzyme from Pseudomonas syringae pv. glycinea has been expressed for the first time under the control of Trichoderma reesei cbh1 promoter in Trichoderma viride. Reverse transcription polymerase chain reaction analysis showed that transformant Y2 produced mRNA of the efe gene. Southern blot analysis showed that there was one copy of efe gene which was integrated into the chromosomal DNA of T. viride. Ethylene production by transformant Y2 was efficiently induced by cellulose, while very low level of ethylene was produced when sodium carboxymethyl cellulose or lactose was used as carbon source. Peptone exerted a much greater stimulatory effect on ethylene production. A high level of ethylene was produced when transformant Y2 was cultured in solid fermentation medium containing wheat straw, indicating that plant wastes could be directly converted to ethylene by the recombinant filamentous fungus.

Keywords

Ethylene Ethylene-forming enzyme (EFE) cbh1 promoter Trichoderma viride Pseudomonas syringae 

Notes

Acknowledgements

We wish to thank Dr. Qun He for discussions. This work was supported by China high Technology (863) Project (Grant No. 2006AA10A213).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Li Tao
    • 1
  • Hong-Jun Dong
    • 1
  • Xi Chen
    • 1
  • San-Feng Chen
    • 1
  • Tian-Hong Wang
    • 2
  1. 1.National Key Laboratory for Agrobiotechnology, College of Biological Sciences and Center for Biomass EngineeringChina Agricultural UniversityBeijingPeople’s Republic of China
  2. 2.The State Key Laboratory of Microbial TechnologyShandong UniversityJinanChina

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