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Optimization of culture conditions and scale-up to plant scales for teicoplanin production by Actinoplanes teichomyceticus

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Abstract

This report describes the optimization of culture conditions for teicoplanin production by Actinoplanes teichomyceticus KCCM-10601, an identified high-teicoplanin-producing strain (US 2006/0134757 A1). Among the conditions tested, temperature, pH, and the dissolved oxygen tension (DOT) were key factors affecting teicoplanin production. When the temperature, pH, and DOT were controlled at 34 °C, 7.0 and 20–30%, respectively, a dry-cell weight of 42.8 g l−1 and a teicoplanin production of 2.9 g l−1 were obtained after 120 h of batch culture, corresponding to a specific teicoplanin content of 67.8 mg g-DCW−1. Teicoplanin production was scaled-up from a laboratory scale (7-l fermenter) to a pilot scale (300 l) and a plant scale (5,000 l) using the impeller tip velocity (V tip) as a scale-up parameter. Teicoplanin production at the laboratory scale was similar to those at the pilot and plant scales. This is the highest report of pilot- and plant-scale production of teicoplanin.

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Acknowledgment

This work was supported by a grant from the Korea Sanhak Foundation (2006 A013–0009).

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Correspondence to Jung-Kul Lee.

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Jung, H., Kim, S., Prabhu, P. et al. Optimization of culture conditions and scale-up to plant scales for teicoplanin production by Actinoplanes teichomyceticus . Appl Microbiol Biotechnol 80, 21 (2008). https://doi.org/10.1007/s00253-008-1530-2

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Keywords

  • Actinoplanes teichomyceticus
  • DOT
  • Impeller tip velocity
  • Optimization
  • Scale-up
  • Teicoplanin