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Determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity

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Abstract

Beech and pine wood blocks were treated with 1,3-dimethylol-4,5-dihydroxyethylen urea (DMDHEU) to increasing weight percent gains (WPG). The resistance of the treated specimens against Trametes versicolor and Coniophora puteana, determined as mass loss, increased with increasing WPG of DMDHEU. Metabolic activity of the fungi in the wood blocks was assessed as total esterase activity (TEA) based on the hydrolysis of fluorescein diacetate and as heat or energy production determined by isothermal micro-calorimetry. Both methods revealed that the fungal activity was related with the WPG and the mass loss caused by the fungi. Still, fungal activity was detected even in wood blocks of the highest WPG and showed that the treatment was not toxic to the fungi. Energy production showed a higher consistency with the mass loss after decay than TEA; higher mass loss was more stringently reflected by higher heat production rate. Heat production did not proceed linearly, possibly due to the inhibition of fungal activity by an excess of carbon dioxide.

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Acknowledgements

The authors are grateful to the German Science Foundation (DFG) for supporting this project. They would also like to thank Prof. Jody Jellison and Prof. Barry Goodell for fruitful discussions and for their sound advice.

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Correspondence to Carsten Mai.

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Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Verma, P., Dyckmans, J., Militz, H. et al. Determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity. Appl Microbiol Biotechnol 80, 125 (2008). https://doi.org/10.1007/s00253-008-1525-z

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Keywords

  • Brown rot
  • Fungal activity
  • Micro-calorimetry
  • Total esterase activity
  • White rot
  • Wood modification