Applied Microbiology and Biotechnology

, Volume 79, Issue 4, pp 657–661 | Cite as

Intergeneric coaggregation of strains isolated from phenol-degrading aerobic granules

Environmental Biotechnology


This work aims at exploring the intergeneric coaggregation of the pairs of strains, Acinetobacter calcoaceticus I6 and Bacillus thuringiensis I2 or Candida tropicalis I9 (with GenBank accession numbers EU250016, EU036759, and DQ515822) isolated from phenol-degrading aerobic granules. The I2 and I6 are functionally similar stains, while the I6 and I9 are functionally dissimilar strains. The lectin–saccharide interaction controlled the coaggregation of both the I2+I6 and I6+I9 pairs, with the protein adhesin being associated with the strain I6, and the complementary galactosamine-like or fucose-like sugar receptor with the strain I2 or I9, respectively. The rod-like I2 cells bridged the clusters of I2 or I6 cells to form aggregates, while the small I6 cells attached on and modified the surface of I9 to form aggregates.


Coaggregation Aerobic granules Lectin–saccharide interaction 


  1. Adav SS, Chen MY, Lee DJ, Ren NQ (2007a) Degradation of phenol by Acinetobactor strain isolated from aerobic granules. Chemosphere 67:1566–1572CrossRefGoogle Scholar
  2. Adav SS, Lee DJ (2008a) Physiological characterization and interactions of isolates in phenol degrading aerobic granules. Appl Microbiol Biotechnol DOI 10.1007/s00253-008-1370-0
  3. Adav SS, Lee DJ (2008b) Single-culture aerobic granules with Acinetobacter calcoaceticus. Appl Microbiol Biotechnol 78:551–557Google Scholar
  4. APHA (1998) The standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington, DC, USAGoogle Scholar
  5. Beun JJ, Hendriks A, van Loosdrecht MCM, Morgenroth E, Wilderer PA, Heijnen JJ (1999) Aerobic granulation in a sequencing batch reactor. Water Res 33:2283–2290CrossRefGoogle Scholar
  6. Beun JJ, van Loosdrecht MCM, Heijnen JJ (2002) Aerobic granulation in a sequencing batch airlift reactor. Water Res 36:702–712CrossRefGoogle Scholar
  7. Buswell CM, Herlihy YM, Marsh PD, Keevil CW, Leach SA (1997) Coaggregation amongst aquatic biofilm bacteria. J Appl Microbiol 83:477–484CrossRefGoogle Scholar
  8. Grenier D (1992) Nutritional interactions between two suspected periodontopathogens, Treponema denticola and Porphyromonas gingivalis. Infect Immun 60:5298–5301Google Scholar
  9. Jiang HL, Tay JH, Maszenan AM, Tay STL (2004) Bacterial diversity and function of aerobic granules engineered in a sequencing batch reactor for phenol degradation. Appl Environ Microbiol 70:6767–6775CrossRefGoogle Scholar
  10. Jiang HL, Tay JH, Maszenan AM, Tay STL (2006a) Enhanced phenol biodegradation and aerobic granulation by two coaggregating bacterial strains. Environ Sci Technol 40:6137–6142Google Scholar
  11. Jiang HL, Tay STL, Maszenan AM, Tay JH (2006b) Physiological traits of bacterial strains isolated from phenol-degrading aerobic granules. FEMS Microbiol Ecol 57:182–191Google Scholar
  12. Jiang HL, Maszenan AM, Tay JH (2007) Bioaugmentation and coexistence of two functionally similar bacterial strains in aerobic granules. Appl Microbiol Biotechnol 75:1191–1200CrossRefGoogle Scholar
  13. Liu Y, Tay JH (2002) The essential role of hydrodynamic shear force in the formation of biofilm and granular sludge. Water Res 36:1653–65CrossRefGoogle Scholar
  14. Liu Y, Wang ZW, Qin L, Liu YQ, Tay JH (2005) Selection pressure-driven aerobic granulation in a sequencing batch reactor. Appl Microbiol Biotechnol 67:26–32Google Scholar
  15. Malik A, Sakamoto M, Hanazaki S, Osawa M, Suzuki T, Tochigi M, Kakii K (2003) Coaggregation among nonflocculating bacteria isolated from activated sludge. Appl Environ Microbiol 69:6056–6063CrossRefGoogle Scholar
  16. Palmer RJ, Kazmerzak K, Hansen MC, Kolenbrander PE (2001) Mutualism versus independence: strategies of mixed-species oral biofilms in vitro using saliva as the sole nutrient source. Infect Immun 69:5794–5804CrossRefGoogle Scholar
  17. Qin L, Liu Y, Tay JH (2004a) Effect of settling time on aerobic granulation in sequencing batch reactor. Biochem Eng J 21:47–52CrossRefGoogle Scholar
  18. Qin L, Tay JH, Liu Y (2004b) Selection pressure is a driving force of aerobic granulation in sequencing batch reactors. Process Biochem 39:579–584CrossRefGoogle Scholar
  19. Rickard AH, Gilbert P, Handley PS (2004) Influence of growth environment on coaggregation between freshwater biofilm bacteria. J Appl Microbiol 96:1367–1373CrossRefGoogle Scholar
  20. Su KZ, Yu HQ (2005) Formation and characterization of aerobic granules in a sequencing batch reactor treating soybean-processing wastewater. Environ Sci Technol 39:2818–28CrossRefGoogle Scholar
  21. Tay JH, Liu QS, Liu Y (2001a) The effects of shear force on the formation, structure and metabolism of aerobic granules. Appl Microbiol Biotechnol 57:227–233CrossRefGoogle Scholar
  22. Tay JH, Liu QS, Liu Y (2001b) The role of cellular polysaccharides in the formation and stability of aerobic granules. Lett Appl Microbiol 33:222–226CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Chemical EngineeringNational Taiwan UniversityTaipeiTaiwan
  2. 2.Department of Chemical Engineering, R&D Center of Membrane TechnologyChung Yuan Christian UniversityChungliTaiwan

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