Applied Microbiology and Biotechnology

, Volume 78, Issue 6, pp 991–995 | Cite as

Overexpression of yeast S-adenosylmethionine synthetase metK in Streptomyces actuosus leads to increased production of nosiheptide

  • Xincheng Zhang
  • Meiqing Fen
  • Xunlong Shi
  • Linquan BaiEmail author
  • Pei ZhouEmail author
Applied Genetics and Molecular Biotechnology


S-Adenosylmethionine (SAM) is synthesized via the metabolic reaction involving adenosine triphosphate and l-methionine that is catalyzed by the enzyme S-adenosyl-l-methionine synthetase (SAM-s) and encoded by the gene metK. In the present study, metK with the absence of introns from Saccharomyces cerevisiae was introduced into Streptomyces actuosus, a nosiheptide (Nsh) producer. Intracellular SAM levels were determined by high-pressure liquid chromatography. Through optimizing the nutrient content of the medium, it was shown that increased SAM production induced by the overexpression of SAM-s leads to an increase in the intracellular cysteine pool and overproduction of Nsh in S. actuosus. This investigation shows that increased SAM promotes the elevated production of the non-ribosomal thiopeptide Nsh in Streptomyces sp.


Streptomyces actuosus S-Adenosylmethionine metK Cysteine Nosiheptide 


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.School of PharmacyFudan UniversityShanghaiChina
  2. 2.Laboratory of Microbial Metabolism, School of Life Science and BiotechnologyShanghai Jiaotong UniversityShanghaiChina

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