Overexpression of yeast S-adenosylmethionine synthetase metK in Streptomyces actuosus leads to increased production of nosiheptide
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S-Adenosylmethionine (SAM) is synthesized via the metabolic reaction involving adenosine triphosphate and l-methionine that is catalyzed by the enzyme S-adenosyl-l-methionine synthetase (SAM-s) and encoded by the gene metK. In the present study, metK with the absence of introns from Saccharomyces cerevisiae was introduced into Streptomyces actuosus, a nosiheptide (Nsh) producer. Intracellular SAM levels were determined by high-pressure liquid chromatography. Through optimizing the nutrient content of the medium, it was shown that increased SAM production induced by the overexpression of SAM-s leads to an increase in the intracellular cysteine pool and overproduction of Nsh in S. actuosus. This investigation shows that increased SAM promotes the elevated production of the non-ribosomal thiopeptide Nsh in Streptomyces sp.
KeywordsStreptomyces actuosus S-Adenosylmethionine metK Cysteine Nosiheptide
- Mocek U, Chen LC, Keller PJ, Houck DR, Beale JM, Floss HG (1989) Proton and carbon-13 NMR assignments of the thiopeptide antibiotic nosiheptide. J Antibiotics 42:1643–1648Google Scholar
- Nakajima A, Wada K, Katayama K, Saubermann L, Osawa E, Nagase H, Ueno N, Matsuhashi N, Aburatani H (2002) Gene expression profile after peroxisome proliferator activator receptor-gamma ligand administration in dextran sodium sulfate mice. J Gastroenterol 37(14):62–66Google Scholar
- Williamson JM, Meyer R, Inamine E (1985) Reverse transsulfuration and its relationship to thienamycin biosynthesis in Streptomyces cattleya. Antimicrob Agents Chemother 28(4):478Google Scholar