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Applied Microbiology and Biotechnology

, Volume 78, Issue 5, pp 821–826 | Cite as

A low-sodium-salt formulation for the fermentation of salinosporamides by Salinispora tropica strain NPS21184

  • Ginger Tsueng
  • Kin S. Lam
Applied Microbial and Cell Physiology

Abstract

In this paper, we described the development of a potassium-chloride-based-salt formulation containing low sodium concentrations (5.0 to 11 mM) to support the growth of Salinispora tropica strain NPS21184 and its production of salinosporamide A (NPI-0052). The sodium present in the media was essentially derived from the complex nitrogen sources Hy Soy, yeast extract, and peptone used in the media. We demonstrated that good growth rate and yield of S. tropica strain NPS21184 were detected in both agar and liquid media containing the potassium-chloride-based-salt formulation with sodium concentration as low as 5.0 mM, significantly less than the critical seawater-growth requirement concentration of 50 mM sodium for a marine microorganism. We also observed good production of NPI-0052 (176 to 243 mg/l) by S. tropica strain NPS21184 grown in production media containing the potassium chloride-based-salt formulation. The production of deschloro analog, salinosporamide B (NPI-0047), was significantly lower in the low-sodium-salt-formulation medium than in the high-sodium-salt-formulation media. We demonstrated that while S. tropica strain NPS21184 is a novel marine actinomycete that requires high salt content for growth, it does not require sodium-chloride-based seawater-type media for growth and production of NPI-0052.

Keywords

Salinosporamide A NPI-0052 Salinispora tropica Low-sodium-salt formulation Non-saline fermentation Non-seawater-growth requirement 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Nereus Pharmaceuticals, Inc.San DiegoUSA

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