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Applied Microbiology and Biotechnology

, Volume 78, Issue 2, pp 227–232 | Cite as

Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture

  • Jorge QuillaguamánEmail author
  • Thuoc Doan-Van
  • Héctor Guzmán
  • Daniel Guzmán
  • Javier Martín
  • Akaraonye Everest
  • Rajni Hatti-Kaul
Biotechnological products and process engineering

Abstract

High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l−1). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH4Cl and K2HPO4 on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH4Cl and 0.22% (w/v) K2HPO4 and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l−1, respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH4Cl, and K2HPO4 were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l−1, PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l−1 h−1.

Keywords

Halomonas boliviensis Moderate halophile Fed-batch cultivation PHB production Glutamate 

Notes

Acknowledgement

The authors would like to thank the Swedish International Development Cooperation Agency (Sida) for supporting this work.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Jorge Quillaguamán
    • 1
    Email author
  • Thuoc Doan-Van
    • 1
    • 2
  • Héctor Guzmán
    • 1
    • 3
  • Daniel Guzmán
    • 1
    • 3
  • Javier Martín
    • 1
  • Akaraonye Everest
    • 1
  • Rajni Hatti-Kaul
    • 1
  1. 1.Department of Biotechnology, Center for Chemistry and Chemical EngineeringLund UniversityLundSweden
  2. 2.Department of Micro-biotechnology, Faculty of Agro-BiologyHanoi University of EducationHanoiVietnam
  3. 3.Centro de Biotecnología, Facultad de Ciencias y TecnologíaUniversidad Mayor de San SimónCochabambaBolivia

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