Applied Microbiology and Biotechnology

, Volume 77, Issue 6, pp 1359–1365 | Cite as

Pathway, inhibition and regulation of methyl tertiary butyl ether oxidation in a filamentous fungus, Graphium sp.

  • Kristin M. Skinner
  • Adriana Martinez-Prado
  • Michael R. Hyman
  • Kenneth J. Williamson
  • Lynda M. Ciuffetti
Applied Microbial and Cell Physiology

Abstract

The filamentous fungus Graphium sp. (ATCC 58400) co-metabolically oxidizes the gasoline oxygenate methyl tertiary butyl ether (MTBE) after growth on gaseous n-alkanes. In this study, the enzymology and regulation of MTBE oxidation by propane-grown mycelia of Graphium sp. were further investigated and defined. The trends observed during MTBE oxidation closely resembled those described for propane-grown cells of the bacterium Mycobacterium vaccae JOB5. Propane-grown mycelia initially oxidized the majority (∼95%) of MTBE to tertiary butyl formate (TBF), and this ester was biotically hydrolyzed to tertiary butyl alcohol (TBA). However, unlike M. vaccae JOB5, our results collectively suggest that propane-grown mycelia only have a limited capacity to degrade TBA. None of the products of MTBE exerted a physiologically relevant regulatory effect on the rate of MTBE or propane oxidation, and no significant effect of TBA was observed on the rate of TBF hydrolysis. Together, these results suggest that the regulatory effects of MTBE oxidation intermediates proposed for MTBE-degrading organisms such as Mycobacterium austroafricanum are not universally relevant mechanisms for MTBE-degrading organisms. The results of this study are discussed in terms of their impact on our understanding of the diversity of aerobic MTBE-degrading organisms and pathways and enzymes involved in these processes.

Keywords

Methyl tertiary butyl alcohol Cometabolism Graphium sp. Tertiary butyl alcohol Tertiary butyl formate Alkane monooxygenase 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Kristin M. Skinner
    • 1
  • Adriana Martinez-Prado
    • 2
    • 3
  • Michael R. Hyman
    • 4
  • Kenneth J. Williamson
    • 2
  • Lynda M. Ciuffetti
    • 1
  1. 1.Department of Botany and Plant PathologyOregon State UniversityCorvallisUSA
  2. 2.Department of Civil, Construction and Environmental EngineeringOregon State UniversityCorvallisUSA
  3. 3.Departamento de Ingeniería Química y BioquímicaInstituto Tecnológico de DurangoDurangoMexico
  4. 4.Department of MicrobiologyNorth Carolina State UniversityRaleighUSA

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