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Applied Microbiology and Biotechnology

, Volume 77, Issue 6, pp 1219–1224 | Cite as

Production of isopropanol by metabolically engineered Escherichia coli

  • Toru Jojima
  • Masayuki Inui
  • Hideaki YukawaEmail author
Biotechnological Products and Process Engineering

Abstract

A genetically engineered strain of Escherichia coli JM109 harboring the isopropanol-producing pathway consisting of five genes encoding four enzymes, thiolase, coenzyme A (CoA) transferase, acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824, and primary–secondary alcohol dehydrogenase from C. beijerinckii NRRL B593, produced up to 227 mM of isopropanol from glucose under aerobic fed-batch culture conditions. Acetate production by the engineered strain was approximately one sixth that produced by a control E. coli strain bearing an expression vector without the clostridial genes. These results demonstrate a functional isopropanol-producing pathway in E. coli and consequently carbon flux from acetyl-CoA directed to isopropanol instead of acetate. This is the first report on isopropanol production by genetically engineered microorganism under aerobic culture conditions.

Keywords

Isopropanol  Escherichia coli Clostridium acetobutylicum Clostridium beijerinckii Metabolic engineering 

Notes

Acknowledgment

We thank C. A. Omumasaba (RITE) for helpful comments on the manuscript.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Research Institute of Innovative Technology for the Earth (RITE)KizugawaJapan

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