Applied Microbiology and Biotechnology

, Volume 77, Issue 4, pp 763–769 | Cite as

Influence of culture conditions on glutathione production by Saccharomyces cerevisiae

  • Lucielen Oliveira Santos
  • Tatiane Araujo Gonzales
  • Beatriz Torsani Úbeda
  • Ranulfo Monte Alegre
Biotechnological Products and Process Engineering

Abstract

A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20–30°C), agitation rate (100–300 rpm), initial pH (5.0–7.0), inoculum concentration (5–15%), and glucose concentration (30–70 g/l) for glutathione (GSH) production in shake-flask culture by Saccharomyces cerevisiae ATCC 7754. By a FFD (25–2), the agitation rate, temperature, and pH were found to be significant factors for GSH production. In CCRD (22) was obtained a second-order model equation, and the percent of variation explained by the model was 95%. The results showed that the optimal culture conditions were agitation rate, 300 rpm; temperature, 20°C; initial pH, 5; glucose, 54 g/l; and inoculum concentration, 5%. The highest GSH concentration (154.5 mg/l) was obtained after 72 h of fermentation.

Keywords

Glutathione Saccharomyces cerevisiae Culture optimization Experimental design 

Notes

Acknowledgements

The authors would like to thank the financial support from The National Council for Scientific and Technological Development (CNPq) of Brazil.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Lucielen Oliveira Santos
    • 1
  • Tatiane Araujo Gonzales
    • 1
  • Beatriz Torsani Úbeda
    • 1
  • Ranulfo Monte Alegre
    • 1
  1. 1.Department of Food Engineering, College of Food EngineeringUNICAMP (State University of Campinas)CampinasBrazil

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