Applied Microbiology and Biotechnology

, Volume 77, Issue 3, pp 533–541 | Cite as

Direct production of L-lysine from raw corn starch by Corynebacterium glutamicum secreting Streptococcus bovis α-amylase using cspB promoter and signal sequence

Biotechnological Products and Process Engineering

Abstract

Corynebacterium glutamicum is an important microorganism in the industrial production of amino acids. We engineered a strain of C. glutamicum that secretes α-amylase from Streptococcus bovis 148 (AmyA) for the efficient utilization of raw starch. Among the promoters and signal sequences tested, those of cspB from C. glutamicum possessed the highest expression level. The fusion gene was introduced into the homoserine dehydrogenase gene locus on the chromosome by homologous recombination. L-Lysine fermentation was conducted using C. glutamicum secreting AmyA in the growth medium containing 50 g/l of raw corn starch as the sole carbon source at various temperatures in the range 30 to 40°C. Efficient L-lysine production and raw starch degradation were achieved at 34 and 37°C, respectively. The α-amylase activity using raw corn starch was more than 2.5 times higher than that using glucose as the sole carbon source during L-lysine fermentation. AmyA expression under the control of cspB promoter was assumed to be induced when raw starch was used as the sole carbon source. These results indicate that efficient simultaneous saccharification and fermentation of raw corn starch to L-lysine were achieved by C. glutamicum secreting AmyA using the cspB promoter and signal sequence.

Keywords

Corynebacterium glutamicum Raw starch cspB promoter α-Amylase 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Toshihiro Tateno
    • 1
  • Hideki Fukuda
    • 2
  • Akihiko Kondo
    • 3
  1. 1.Department of Molecular Science and Material Engineering, Graduate School of Science and TechnologyKobe UniversityKobeJapan
  2. 2.Organization of Advanced Science and TechnologyKobe UniversityKobeJapan
  3. 3.Department of Chemical Science and Engineering, Graduate school of EngineeringKobe UniversityKobeJapan

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