Applied Microbiology and Biotechnology

, Volume 77, Issue 2, pp 339–346

Hyaluronic acid production by recombinant Lactococcus lactis

Applied Genetics and Molecular Biotechnology


Microbial hyaluronic acid (HA), commonly produced by pathogenic Streptococcus, was made possible to be produced by a generally recognized as safe Lactococcus lactis by coexpressing HA synthase and uridine diphosphate–glucose dehydrogenase (UDP-GlcDH) of Streptococcus equi subsp. zooepidemicus in a nisin-controlled expression (NICE) system. With scarce expressed HA synthase alone, the constructed recombinant L. lactis (LL-NA) strain could produce HA with a concentration about 0.08 g/l in the M17 medium supplemented with 1% (w/v) glucose. In contrast to HA synthase, UDP-GlcDH of Streptococcus could be overexpressed in the NICE system. With coexpression of heterologous UDP-GlcDH with HA synthase, the constructed LL-NAB strain grew slightly slower to a concentration about 10% lower that of the LL-NA strain. However, the HA concentration produced was enhanced about eightfold to 0.65 g/l.


Hyaluronic acid Nisin NICE Lactococcus lactis HA synthase expression 


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of Chemical EngineeringNational Taiwan University of Science and TechnologyTaipeiTaiwan

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