The pSKC2 cosmid, which has 32 kb and 28 open-reading frames, was isolated from Streptomyces kanamyceticus ATCC12853 as the gene cluster of kanamycin. This gene cluster includes the minimal biosynthetic genes of kanamycin with the resistance and regulatory genes. It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid production of the aminoglycosides antibiotic. The isolated compound was analyzed by electrospray ionization–mass spectrometry, liquid chromatography–mass spectrometry, high-performance liquid chromatography, and tandem mass spectrometry and shows a molecular weight of 485 as kanamycin A.
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This work was supported by Ministry of Commerce, Industry and Energy (grant no. 10023194) and by a grant (no. 20050401034682) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.
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Thapa, L.P., Oh, T., Lee, H.C. et al. Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003. Appl Microbiol Biotechnol 76, 1357–1364 (2007). https://doi.org/10.1007/s00253-007-1096-4
- Heterologous expression
- Streptomyces kanamyceticus ATCC 12853
- Streptomyces venezuelae YJ003