Applied Microbiology and Biotechnology

, Volume 76, Issue 2, pp 467–472 | Cite as

Characterization of technetium(vII) reduction by cell suspensions of thermophilic bacteria and archaea

  • Nikolay A. Chernyh
  • Sergei N. Gavrilov
  • Vladimir V. Sorokin
  • Konstantin E. German
  • Claire Sergeant
  • Monique Simonoff
  • Frank Robb
  • Alexander I. SlobodkinEmail author
Applied Microbial and Cell Physiology


Washed cell suspensions of the anaerobic hyperthermophilic archaea Thermococcus pacificus and Thermoproteus uzoniensis and the anaerobic thermophilic gram-positive bacteria Thermoterrabacterium ferrireducens and Tepidibacter thalassicus reduced technetium [99Tc(VII)], supplied as soluble pertechnetate with molecular hydrogen as an electron donor, forming highly insoluble Tc(IV)-containing grayish-black precipitate. Apart from molecular hydrogen, T. ferrireducens reduced Tc(VII) with lactate, glycerol, and yeast extract as electron donors, and T. thalassicus reduced it with peptone. Scanning electron microscopy and X-ray microanalysis of cell suspensions of T. ferrireducens showed the presence of Tc-containing particles attached to the surfaces of non-lysed cells. This is the first report on the reduction in Tc(VII) by thermophilic microorganisms of the domain Bacteria and by archaea of the phylum Euryarchaeota.


Technetium reduction Tc(VII) reduction Thermophilic microorganisms Radionuclide immobilization 



This work was supported by CNRS (Programme International de Coopération Scientifique no. 2730 and grant 04-03-22000-CNRS from the Russian Foundation for Basic Research), by University of Bordeaux I, CRDF grant RB-2-2379-MO-02C, and by the Program ‘Molecular and Cell Biology’ of the Russian Academy of Sciences. Studies in the Robb laboratory were supported by NSF Grants MCB 0238337. We thank A.V. Lebedinsky for the valuable discussions of the manuscript.


  1. Bonch-Osmolovskaya EA, Miroshnichenko ML, Kostrikina NA, Chernch NA, Zavarzin GA (1991) Thermoproteus uzoniensis sp. nov., a new extremely thermophilic archaebacterium from Kamchatka continental hot springs. Arch Microbiol 154:556–559Google Scholar
  2. De Luca G, de Philip P, Dermoun Z, Rousset M, Vermeglio A (2001) Reduction of technetium(VII) by Desulfovibrio fructosovorans is mediated by the nickel–iron hydrogenase. Appl Environ Microbiol 67:4583–4587CrossRefGoogle Scholar
  3. Francis AJ, Dodge CJ, Meinken GE (2002) Biotransformation of pertechnetate by Clostridia. Radiochim Acta 90:791–797CrossRefGoogle Scholar
  4. Gavrilov SN, Bonch-Osmolovskaya EA, Slobodkin AI (2003) Physiology of organotrophic and lithotrophic growth of the thermophilic iron-reducing bacteria Thermoterrabacterium ferrireducens and Thermoanaerobacter siderophilus. Microbiology 72:132–137CrossRefGoogle Scholar
  5. Henstra AM, Stams AJM (2004) Novel physiological features of Carboxydothermus hydrogenoformans and Thermoterrabacterium ferrireducens. Appl Environ Microbiol 70:7236–7240CrossRefGoogle Scholar
  6. Kashefi K and Lovley DR (2000) Reduction of Fe(III), Mn(IV), and toxic metals at 100°C by Pyrobaculum islandicum. Appl Environ Microbiol 66:1050–1056CrossRefGoogle Scholar
  7. Khijniak TV, Medvedeva-Lyalikova NN, Simonoff M (2003) Reduction of pertechnetate by haloalkaliphilic strains of Halomonas. FEMS Microbiol Ecol 44:109–115CrossRefGoogle Scholar
  8. Khijniak TV, Slobodkin AI, Coker V, Renshaw JC, Livens FR, Bonch-Osmolovskaya EA, Birkeland N-K, Medvedeva-Lyalikova NN, Lloyd JR (2005). Reduction of uranium(VI) phosphate during growth of the thermophilic bacterium Thermoterrabacterium ferrireducens. Appl Environ Microbiol 71:6423–6426CrossRefGoogle Scholar
  9. Lloyd JR, Macaskie LE (1996) A novel PhosphorImager based technique for monitoring the microbial reduction of technetium. Appl Environ Microbiol 62:578–582Google Scholar
  10. Lloyd JR, Cole JA, Macaskie LE (1997) Reduction and removal of heptavalent technetium from solution by Esherichia coli. J Bacteriol 179:2014–2021Google Scholar
  11. Lloyd JR, Nolting H-F, Sole VA, Bosecker K, Macaskie LE (1998) Technetium reduction and precipitation by sulfate-reducing bacteria. Geomicrobiol J 15:45–58CrossRefGoogle Scholar
  12. Lloyd JR, Chesnes J, Glasauer S, Bunker DJ, Livens FR, Lovley DR (2002) Reduction of actinides and fission products by Fe(III)-reducing bacteria. Geomicrobiol J 19:103–120CrossRefGoogle Scholar
  13. Lowry OH., Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin–phenol reagents. J Biol Chem 193:265–275Google Scholar
  14. Lyalikova NN, Khizhnyak TV (1996) Reduction of heptavalent technetium by acidophilic bacteria of the genus Thiobacillus. Microbiology 65:468–473Google Scholar
  15. Macaskie LE (1991) The application of biotechnology to the treatment of wastes produced from nuclear fuel cycle: biodegradation and bioaccumulation as a means of treating radionuclide-containing streams. Crit Rev Biotechnol 11:41–112Google Scholar
  16. Miroshnichenko ML, Gongadze GM, Rainey FA, Kostyukova AS, Lysenko AM, Chernyh NA, Bonch-Osmolovskaya EA. (1998) Thermococcus gorgonarius sp. nov. and Thermococcus pacificus sp. nov.: heterotrophic extremely thermophilic archaea from New Zealand submarine hot vents. Int J Syst Bacteriol 48:23–29Google Scholar
  17. Miroshnichenko ML, Hippe H, Stackebrandt E, Kostrikina NA, Chernyh NA, Jeanthon C, Nazina TN, Belyaev SS, Bonch-Osmolovskaya EA (2001) Isolation and characterization of Thermococcus sibiricus sp. nov. from a Western Siberia high-temperature oil reservoir. Extremophiles 5:85–91CrossRefGoogle Scholar
  18. Mulyukin AL, Sorokin VV, Loiko NG, Suzina NE, Duda VI, Vorob’eva EA, El’-Registan GI (2002) Comparative study of the elemental composition of vegetative and resting microbial cells. Microbiology 71:31–40CrossRefGoogle Scholar
  19. Peretruchin VF, Khijniak TV, Lyalikova NN, German KE (1996) Biosorbtion of technetium-99 and some actinides by bottom sediments from lake Beloe Kosino, Moscow Region. Radiochemistry 38:471–475 (in Russian)Google Scholar
  20. Simonoff M, Khijniak T, Sergeant C, Vesvres MH, Pravikoff MS, Leclerc-Cessac E, Echevarria G, Denys S (2003) Technetium species induced in maize as measured by Phosphorimager.J Environ Radioact 70:139–154CrossRefGoogle Scholar
  21. Slobodkin AI, Reysenbach A-L, Strutz N, Dreier M, Wiegel J (1997) Thermoterrabacterium ferrireducens gen. nov., sp. nov. a thermophilic anaerobic, dissimilatory Fe(III)-reducing bacterium from a continental hot spring. Int J Syst Bacteriol 47:541–547CrossRefGoogle Scholar
  22. Slobodkin A, Campbell B, Cary SC, Bonch-Osmolovskaya E, Jeanthon C (2001) Evidence for the presence of thermophilic Fe(III)-reducing microorganisms in deep-sea hydrothermal vents at 13°N (East Pacific Rise). FEMS Microbiol Ecol 36:235–243Google Scholar
  23. Slobodkin AI, Tourova TP, Kostrikina NA, Chernyh NA, Bonch-Osmolovskaya EA, Jeanthon C, Jones BE (2003) Tepidibacter thalassicus gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 53:1131–1134CrossRefGoogle Scholar
  24. Sorokin VV, Mulyukin AL, Vorobyova EA, Suzina NE, Duda VI, El-Registan GI (2003) Detection of microbial cells and preliminary estimation of their physiological state by X-ray microanalysis. In: Hoover et al (eds) Instruments, methods, and missions for astrobiology VI. Proc SPIE Int Soc Opt Eng 4939:219–223Google Scholar
  25. Wildung RE, McFadden KM, Garland TR (1979) Technetium sources and behavior in the environment. J Environ Qual 8:156–161CrossRefGoogle Scholar
  26. Wildung RE, Gorby YA, Krupka KM, Hess NJ, Li SW, Plymale AE, McKinley JP, Fredrickson JK (2000) Effect of electron donor and solution chemistry on products of dissimilatory reduction of technetium by Shewanella putrefaciens. Appl Environ Microbiol 66:2451–2460CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Nikolay A. Chernyh
    • 1
  • Sergei N. Gavrilov
    • 1
  • Vladimir V. Sorokin
    • 1
  • Konstantin E. German
    • 2
  • Claire Sergeant
    • 3
  • Monique Simonoff
    • 3
  • Frank Robb
    • 4
  • Alexander I. Slobodkin
    • 1
    Email author
  1. 1.Winogradsky Institute of MicrobiologyRussian Academy of SciencesMoscowRussia
  2. 2.Institute of Electrochemistry and Physical ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Laboratoire de Chimie Nucleaire Analytique et BioenvironnementaleCNRS-Universite de Bordeaux I, UMR 5084Gradignan CedexFrance
  4. 4.Center for Marine BiotechnologyBaltimoreUSA

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