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Applied Microbiology and Biotechnology

, Volume 76, Issue 1, pp 61–65 | Cite as

Microbial asymmetric oxidation of 2-butyl-1,3-propanediol

  • K. Mitsukura
  • T. Uno
  • T. Yoshida
  • T. Nagasawa
Biotechnological Products and Process Engineering

Abstract

Microbial asymmetric oxidation of 2-butyl-1,3-propanediol was investigated for an efficient synthesis of S- and R-enantiomers of 2-hydroxymethylhexanoic acid (2-HMHA). From an intensive survey of the stocked bacterial strains, Acetobacter pasteurianus IAM 12073 and Pseudomonas putida IFO 3738 were found to show the highest S- and R-2-HMHA-producing activity, respectively. Under optimized conditions, A. pasteurianus (351 mg dry cell weight) and P. putida (642 mg dry cell weight) cells produced 12.0 g l−1 S-2-HMHA with 89% enantiomeric excess (e.e.) at 24 h of incubation and 5.1 g l−1 R-2-HMHA with 94% e.e. at 35 h of incubation from 2-butyl-1,3-propanediol.

Keywords

Acetobacter pasteurianus Asymmetric oxidation 2-Butyl-1,3-propanediol 2-Hydroxymethylhexanoic acid Pseudomonas putida 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of Biomolecular Science, Faculty of EngineeringGifu UniversityGifuJapan

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