Characterization of a chitinolytic enzyme from Serratia sp. KCK isolated from kimchi juice
- 199 Downloads
The novel chitinolytic bacterium Serratia sp. KCK, which was isolated from kimchi juice, produced chitinase A. The gene coding for the chitinolytic enzyme was cloned on the basis of sequencing of internal peptides, homology search, and design of degenerated primers. The cloned open reading frame of chiA encodes for deduced polypeptide of 563 amino acid residues with a calculated molecular mass of 61 kDa and appears to correspond to a molecular mass of about 57 kDa, which excluded the signal sequence. The deduced amino acid sequence showed high similarity to those of bacterial chitinases classified as family 18 of glycosyl hydrolases. The chitinase A is an exochitinase and exhibits a greater pH range (5.0–10.0), thermostability with a temperature optimum of 40°C, and substrate range other than Serratia chitinases thus far described. These results suggested that Serratia sp. KCK chitinase A can be used for biotechnological applications with good potential.
KeywordsChitinolytic bacteria and enzyme Chitinase A Kimchi juice
We gratefully acknowledge MetaGenoMik Project of Federal Ministry for Science and Education (BMBF) and Fonds der Chemischen Industrie for generous support.
- Chernin LS, Fuente LDL, Sobolev V, Haran S, Vorgias CE, Oppenheim AB, Chet I (1997) Molecular cloning, structural analysis, and expression in Escherichia coli of a chitinase gene from Enterobacter agglomerans. Appl Environ Microbiol 63:834–839Google Scholar
- Imoto T, Yagishita K (1971) A simple activity measurement of lysozyme. Agric Biol Chem 35:1154–1156Google Scholar
- Keyhani NO, Roseman S (1999) Physiological aspects of chitin catabolism in marine bacteria. Biochim Biophys Acta 1473:108–122Google Scholar
- Ohno T, Armand S, Hata T, Nikaidou N, Henrissat B, Mitsutomi M, Watanabe T (1996) A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037. J Bacteriol 178:5065–5070Google Scholar
- Sakai K, Yokota A, Kurokawa H, Wakayama M, Moriguchi M (1998) Purification and characterization of three thermostable endochitinases of a novel Bacillus strain, MH-1, isolated from chitin-containing compost. Appl Environ Microbiol 64:3397–3402Google Scholar
- Shigemasa Y, Minami S (1995) Application of chitin and chitosan for biomaterials. Biotechnol Genet Eng Rev 13:383–420Google Scholar
- Svitil AL, Chadhain SMN, Moore JA, Kirchman DL (1997) Chitin degradation proteins produced by the marine bacterium Vibrio harveyi growing on different forms of chitin. Appl Environ Microbiol 63:408–413Google Scholar
- Wang SL, Chang WT (1997) Purification and characterization of two bifunctional chitinase/lysozyme extracellulary produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium. Appl Environ Microbiol 63:380–386Google Scholar
- Watanabe T, Kimura K, Sumiya T, Nikaidou N, Suzuki K, Suzuki M, Taiyoji M, Ferrer S, Regue M (1997) Genetic analysis of the chitinase system of Serratia marcescens 2170. J Bacteriol 179:7111–7117Google Scholar