Applied Microbiology and Biotechnology

, Volume 74, Issue 4, pp 754–760 | Cite as

Efficient induction of formate hydrogen lyase of aerobically grown Escherichia coli in a three-step biohydrogen production process

  • Akihito Yoshida
  • Taku Nishimura
  • Hideo Kawaguchi
  • Masayuki Inui
  • Hideaki YukawaEmail author
Biotechnological Products and Process Engineering


A three-step biohydrogen production process characterized by efficient anaerobic induction of the formate hydrogen lyase (FHL) of aerobically grown Escherichia coli was established. Using E. coli strain SR13 (fhlA ++, ΔhycA) at a cell density of 8.2 g/l medium in this process, a specific hydrogen productivity (28.0 ± 5.0 mmol h−1 g−1 dry cell) of one order of magnitude lower than we previously reported was realized after 8 h of anaerobic incubation. The reduced productivity was attributed partly to the inhibitory effects of accumulated metabolites on FHL induction. To avoid this inhibition, strain SR14 (SR13 ΔldhA ΔfrdBC) was constructed and used to the effect that specific hydrogen productivity increased 1.3-fold to 37.4 ± 6.9 mmol h−1 g−1. Furthermore, a maximum hydrogen production rate of 144.2 mmol h−1 g−1 was realized when a metabolite excretion system that achieved a dilution rate of 2.0 h−1 was implemented. These results demonstrate that by avoiding anaerobic cultivation altogether, more economical harvesting of hydrogen-producing cells for use in our biohydrogen process was made possible.


Hydrogen Production Dilution Rate Biohydrogen Proton Motive Force Sodium Formate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank C. A. Omumasaba (Research Institute of Innovative Technology for the Earth) for helpful comments on the manuscript.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Akihito Yoshida
    • 1
    • 2
  • Taku Nishimura
    • 1
  • Hideo Kawaguchi
    • 1
  • Masayuki Inui
    • 1
  • Hideaki Yukawa
    • 1
    Email author
  1. 1.Microbiology Research GroupResearch Institute of Innovative Technology for the EarthKyotoJapan
  2. 2.Research Department II, Advanced Materials Research Laboratories, Corporate Research and Development GroupSharp CorporationNaraJapan

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