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Applied Microbiology and Biotechnology

, Volume 74, Issue 3, pp 601–608 | Cite as

Translation elongation factor 1-α gene from Pichia pastoris: molecular cloning, sequence, and use of its promoter

  • Jungoh Ahn
  • Jiyeon Hong
  • Hyeokweon Lee
  • Myongsoo Park
  • Eungyo Lee
  • Chunsuk Kim
  • Euisung Choi
  • Joonki Jung
  • Hongweon LeeEmail author
Applied Genetics and Molecular Biotechnology

Abstract

The gene encoding translation elongation factor 1-α from the yeast Pichia pastoris was cloned. The gene revealed an open reading frame of 1,380 bp with the potential to encode a polypeptide of 459 amino acids with a calculated mass of 50.1 kDa. The potential of the promoter (P TEF1 ) in P. pastoris was investigated with comparison to the glyceraldehyde-3-phosphate dehydrogenase promoter (P GAP ) by using a bacterial lipase gene as a reporter gene. P TEF1 demonstrated a tighter growth-associated expression mode, improved functioning in the presence of high glucose concentrations, and promoter activities that yielded recombinant protein at levels similar to or in one case greater than P GAP . The sequence of the gene was deposited in GenBank under accession no. EF014948.

Keywords

Pichia pastoris Translation elongation factor Constitutive promoter 

Notes

Acknowledgement

We are grateful to Philekorea Technology (Korea) for technical assistance with the real-time quantitative PCR method.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Jungoh Ahn
    • 1
  • Jiyeon Hong
    • 1
  • Hyeokweon Lee
    • 1
  • Myongsoo Park
    • 1
  • Eungyo Lee
    • 2
  • Chunsuk Kim
    • 1
  • Euisung Choi
    • 3
  • Joonki Jung
    • 2
  • Hongweon Lee
    • 1
    Email author
  1. 1.Biotechnology Process Engineering DepartmentKorea Research Institute of Bioscience and BiotechnologyDaejeonSouth Korea
  2. 2.Biomedical Process Development DepartmentKorea Research Institute of Bioscience and BiotechnologyDaejeonSouth Korea
  3. 3.Microbial Function labKorean Research Institute of Bioscience and BiotechnologyDaejeonSouth Korea

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