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Isolation of a carbon disulfide utilizing Thiomonas sp. and its application in a biotrickling filter

Abstract

The carbon disulfide (CS2)-oxidizing bacterium Thiomonas sp. WZW was enriched and isolated using activated sewage sludge as inoculum. Growth of Thiomonas sp. WZW was observed on CS2, thiosulfate, dimethylsulfide (DMS), dimethyldisulfide (DMDS), and H2S. No growth occurred on dimethylsulfoxide, methanol, acetate, and on complex media with glucose, yeast extract, or tryptone. DMDS-grown cells respired CS2, DMS, and DMDS, while thiosulfate-grown cells did not respire CS2. Chemostat cultures growing on thiosulfate could be rapidly adapted to growth on CS2. Growth was observed between pH 6 and 8. The K s values for CS2, thiosulfate, and sulfide of CS2-grown cells were between 5 and 10 μM. CS2 was inhibitory above 0.3 mM. A lab-scale biotrickling filter with lava stone as carrier material for treatment of CS2-polluted air was inoculated with Thiomonas sp. WZW. A rapid start up (95% removal in 1 week) was obtained at an inlet CS2 concentration of 2 cmol l−1 and an initial space velocity (SV) of 54 h−1. Subsequent thiosulfate addition for a week during start up increased the removal to 99%. The step-wise increase of SV to 130 h−1 and a CS2 concentration to 3 μmol l−1 resulted in a stable performance with a removal efficiency of 95%. Feeding mixtures of volatile sulfur compounds showed simultaneous conversion of H2S, CS2, dimethyldisulfide (DMDS), and DMS, with a preference in this order.

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Aknowledgements

We would like to thank Mike S.M. Jetten, Marcel H. Zandvoort, and Marjan Smeulders for stimulating discussions.

Author information

Correspondence to Huub J. M. Op den Camp.

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Pol, A., van der Drift, C. & Op den Camp, H.J.M. Isolation of a carbon disulfide utilizing Thiomonas sp. and its application in a biotrickling filter. Appl Microbiol Biotechnol 74, 439–446 (2007). https://doi.org/10.1007/s00253-006-0663-4

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Keywords

  • Thiosulfate
  • Space Velocity
  • Mineral Medium
  • DMDS
  • Inlet Concentration