Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli
Transcriptional analysis has been investigated to detect the genes involved in organic solvent tolerance. A time course of gene expression profiles of Escherichia coli after exposure to organic solvents revealed that the expression levels of manX, manY, and manZ genes were strongly upregulated by 13.2-, 10.0-, and 7.0-folds, respectively, after 2 min and then decreased after 10 min. Organic solvent tolerance of E. coli was investigated by inducing overexpression of manX, manY, and manZ genes and manXYZ operon that encode a sugar transporter of the phosphotransferase system. Although the expression of manX, manY, and manZ alone was not effective, the organic solvent tolerance level was increased by the expression of manXYZ. The intracellular hexane level was kept lower in E. coli cells overexpressing manXYZ after 4 h of incubation with hexane.
KeywordsOrganic solvent tolerance DNA microarray manXYZ
This study was carried out as a part of “The Project for Development of a Technological Infrastructure for Industrial Bioprocesses on R&D of New Industrial Science and Technology Frontiers” by the Ministry of Economy, Trade, and Industry (METI) and entrusted by the New Energy and Industrial Technology Development Organization (NEDO). This study was partially supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (no. 17560688).
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