Applied Microbiology and Biotechnology

, Volume 73, Issue 6, pp 1452–1462 | Cite as

Biodegradation of methyl parathion and p-nitrophenol: evidence for the presence of a p-nitrophenol 2-hydroxylase in a Gram-negative Serratia sp. strain DS001

  • Suresh B. Pakala
  • Purushotham Gorla
  • Aleem Basha Pinjari
  • Ravi Kumar Krovidi
  • Rajasekhar Baru
  • Mahesh Yanamandra
  • Mike Merrick
  • Dayananda Siddavattam
Environmental Biotechnology

Abstract

A soil bacterium capable of utilizing methyl parathion as sole carbon and energy source was isolated by selective enrichment on minimal medium containing methyl parathion. The strain was identified as belonging to the genus Serratia based on a phylogram constructed using the complete sequence of the 16S rRNA. Serratia sp. strain DS001 utilized methyl parathion, p-nitrophenol, 4-nitrocatechol, and 1,2,4-benzenetriol as sole carbon and energy sources but could not grow using hydroquinone as a source of carbon. p-Nitrophenol and dimethylthiophosphoric acid were found to be the major degradation products of methyl parathion. Growth on p-nitrophenol led to release of stoichiometric amounts of nitrite and to the formation of 4-nitrocatechol and benzenetriol. When these catabolic intermediates of p-nitrophenol were added to resting cells of Serratia sp. strain DS001 oxygen consumption was detected whereas no oxygen consumption was apparent when hydroquinone was added to the resting cells suggesting that it is not part of the p-nitrophenol degradation pathway. Key enzymes involved in degradation of methyl parathion and in conversion of p-nitrophenol to 4-nitrocatechol, namely parathion hydrolase and p-nitrophenol hydroxylase component “A” were detected in the proteomes of the methyl parathion and p-nitrophenol grown cultures, respectively. These studies report for the first time the existence of a p-nitrophenol hydroxylase component “A”, typically found in Gram-positive bacteria, in a Gram-negative strain of the genus Serratia.

Keywords

Parathion hydrolase p-Nitrophenol hydroxylase component A Serratia sp Biodegradation Catabolomics 

Notes

Acknowledgements

We thank Dr. Reddanna for providing GCMS facility and the University Grants Commission, New Delhi and the Department of Science and Technology, New Delhi for providing financial support to carry out the research work. ABP is a recipient of Junior Research Fellowship from UGC, New Delhi. DS is also a recipient of financial support from Institute of Life Sciences. MM is supported by the Biotechnology and Biological Sciences Research Council, UK.

Supplementary material

253_2006_595_MOESM1_ESM.doc (48 kb)
Fig. 1Mass spectrum identifying p-nitrophenol, a degradation product of methyl parathion (DOC 48 640 KB)
253_2006_595_MOESM2_ESM.doc (42 kb)
Fig. 2Mass spectrum identifying dimethyl thiophosphoric acid, a degradation product of methyl parathion (DOC 43 520 KB)
253_2006_595_MOESM3_ESM.doc (60 kb)
Fig. 3Mass spectrum identifying 4-nitrocatechol, a key intermediate in degradation of PNP (DOC 60 928 KB)
253_2006_595_MOESM4_ESM.doc (95 kb)
Fig. 4Mass spectrum identifying 1,2,4-benzenetriol, a key intermediate in degradation of PNP (DOC 97 280 KB)
253_2006_595_MOESM5_ESM.doc (862 kb)
Fig. 52D analysis of the proteome of Serratia sp. strain DS001 cells grown in a glucose, b methylparathion, and cp-nitrophenol (DOC 882 688 KB)

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Suresh B. Pakala
    • 1
  • Purushotham Gorla
    • 1
  • Aleem Basha Pinjari
    • 1
  • Ravi Kumar Krovidi
    • 2
  • Rajasekhar Baru
    • 2
  • Mahesh Yanamandra
    • 2
  • Mike Merrick
    • 3
  • Dayananda Siddavattam
    • 1
  1. 1.Department of Animal SciencesUniversity of HyderabadHyderabadIndia
  2. 2.Discovery Research, Dr. Reddy’s Laboratories Ltd.HyderabadIndia
  3. 3.Department of Molecular MicrobiologyJohn Innes CentreNorwichUK

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