Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Optimization of nutrient parameters for lovastatin production by Monascus purpureus MTCC 369 under submerged fermentation using response surface methodology

  • 1133 Accesses

  • 102 Citations

Abstract

Lovastatin, an inhibitor of HMG-CoA reductase, was produced by submerged fermentation using Monascus purpureus MTCC 369. Five nutritional parameters screened using Plackett–Burman experimental design were optimized by Box–Behnken factorial design of response surface methodology for lovastatin production in shake flask cultures. Maximum lovastatin production of 351 mg/l were predicted in medium containing 29.59 g/l dextrose, 3.86 g/l NH4Cl, 1.73 g/l KH2PO4, 0.86 g/l MgSO4·7H2O, and 0.19 g/l MnSO4·H2O using response surface plots and point prediction tool of DESIGN EXPERT 7.0 (Statease, USA) software.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2

References

  1. Alberts AW, Chen J, Kuron G, Hunt V, Huff J, Hoffman C, Rothrock J, Lopez M, Joshua H, Harris E, Patchett A, Monaghan R, Currie S, Stapley E, Albers-Schonberg G, Hensens O, Hirshfield J, Hoogsteen K, Liesch J, Springer J (1980) Mevinolin: a highly potent competitive inhibitor of hydroxymethyl glutaryl coenzyme A reductase and cholesterol lowering agent. Proc Natl Acad Sci USA 77:3957–3961

  2. Box GEP, Hunter JS (1957) Multifactor experimental design for exploring response surfaces. Ann Math Stat 28:195–241

  3. Chakravarti R, Sahai V (2002) Optimization of compactin production in chemically defined production medium by Penicillium citrinum using statistical methods. Process Biochem 38:481–486

  4. Chang YC, Lee CL, Pan TM (2006) Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged cultures. Appl Microbiol Biotechnol (in press). DOI https://doi.org/10.1007/s00253-006-0325-6

  5. Demain AL (1999) Pharmaceutically active secondary metabolites of microorganisms. Appl Microbiol Biotechnol 52:455–463

  6. Hajjaj H, Niedberger P, Duboc P (2001) Lovastatin biosynthesis by Aspergillus terreus in a chemically defined medium. Appl Environ Microbiol 67:2596–2604

  7. Hebert PR, Gaziano JM, Chan KS, Henneken CH (1997) Cholesterol lowering with statin drugs, risk of stroke and total mortality-an overview of randomized trials. J Am Med Assoc 278:313–321

  8. Lewis GA, Mathieu D, Phan-Tan-Luu R (1999) Drug and pharmaceutical sciences—pharmaceutical experimental design, vol. 92. Marcel Dekker, Inc., USA

  9. Manzoni M, Rollini M (2002) Biosynthesis and biotechnological production of statins by filamentous fungi and applications of these cholesterol-lowering drugs. Appl Microbiol Biotechnol 58:555–564

  10. Manzoni M, Bergomi S, Rollini M, Cavazzoni V (1999) Production of statins by filamentous fungi. Biotechnol Lett 21:253–257

  11. Samiee SM, Moazami N, Haghighi S, Mohseni FA, Mirdamadi S, Bakhtiari MR (2003) Screening of lovastatin production by filamentous fungi. Iran Biomed J 7:29–33

  12. Su YC, Wang JJ, Lin TT, Pan TM (2003) Production of secondary metabolites, γ-amino butyric acid and monacolin K by Monascus. J Ind Microbiol Biotechnol 30:41–46

  13. Tobert JA (2003) Lovastatin and beyond: the history of the HMG-CoA reductase inhibitors. Nat Rev Drug Discov 2:517–526

  14. Valera HR, Gomes J, Lakshmi S, Gururaja R, Suryanarayan S, Kumar D (2005) Lovastatin production by SSF using Aspergillus flavipes. Enzyme Microb Techol 37:521–526

  15. Wang JJ, Lee CL, Pan TM (2003) Improvement of monacolin K, γ-amino butyric acid and citrinin production ratio as a function of environmental conditions of Monascus purpureus NTU 601. J Ind Microbiol Biotechnol 30:669–676

Download references

Author information

Correspondence to Bibhu Prasad Panda.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sayyad, S.A., Panda, B.P., Javed, S. et al. Optimization of nutrient parameters for lovastatin production by Monascus purpureus MTCC 369 under submerged fermentation using response surface methodology. Appl Microbiol Biotechnol 73, 1054–1058 (2007). https://doi.org/10.1007/s00253-006-0577-1

Download citation

Keywords

  • Lovastatin
  • Monascus purpureus
  • Optimization
  • Response surface methodology