Applied Microbiology and Biotechnology

, Volume 73, Issue 5, pp 1054–1058 | Cite as

Optimization of nutrient parameters for lovastatin production by Monascus purpureus MTCC 369 under submerged fermentation using response surface methodology

  • Sadik Ali Sayyad
  • Bibhu Prasad PandaEmail author
  • Saleem Javed
  • Mohd Ali
Biotechnological Products and Process Engineering


Lovastatin, an inhibitor of HMG-CoA reductase, was produced by submerged fermentation using Monascus purpureus MTCC 369. Five nutritional parameters screened using Plackett–Burman experimental design were optimized by Box–Behnken factorial design of response surface methodology for lovastatin production in shake flask cultures. Maximum lovastatin production of 351 mg/l were predicted in medium containing 29.59 g/l dextrose, 3.86 g/l NH4Cl, 1.73 g/l KH2PO4, 0.86 g/l MgSO4·7H2O, and 0.19 g/l MnSO4·H2O using response surface plots and point prediction tool of DESIGN EXPERT 7.0 (Statease, USA) software.


Lovastatin Monascus purpureus Optimization Response surface methodology 


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Sadik Ali Sayyad
    • 1
  • Bibhu Prasad Panda
    • 1
    Email author
  • Saleem Javed
    • 2
  • Mohd Ali
    • 1
  1. 1.Pharmaceutical Biotechnology Laboratory, Faculty of PharmacyJamia Hamdard (Hamdard University)New DelhiIndia
  2. 2.Molecular Biology Laboratory, Faculty of ScienceJamia Hamdard (Hamdard University)New DelhiIndia

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