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Applied Microbiology and Biotechnology

, Volume 73, Issue 3, pp 691–695 | Cite as

Expression of gentisate 1,2-dioxygenase (gdoA) genes involved in aromatic degradation in two haloarchaeal genera

  • D. J. Fairley
  • G. Wang
  • C. Rensing
  • I. L. Pepper
  • M. J. LarkinEmail author
Applied Microbial and Cell Physiology

Abstract

Gentisate-1,2-dioxygenase genes (gdoA), with homology to a number of bacterial dioxygenases, and genes encoding a putative coenzyme A (CoA)-synthetase subunit (acdB) and a CoA-thioesterase (tieA) were identified in two haloarchaeal isolates. In Haloarcula sp. D1, gdoA was expressed during growth on 4-hydroxybenzoate but not benzoate, and acdB and tieA were not expressed during growth on any of the aromatic substrates tested. In contrast, gdoA was expressed in Haloferax sp. D1227 during growth on benzoate, 3-hydroxybenzoate, cinnamate and phenylpropionate, and both acdB and tieA were expressed during growth on benzoate, cinnamate and phenylpropionate, but not on 3-hydroxybenzoate. This pattern of induction is consistent with these genes encoding steps in a CoA-mediated benzoate pathway in this strain.

Keywords

Haloarchaea Aromatic Catabolism Haloferax Benzoate 3-Hydroxybenzoate 

Notes

Acknowledgements

This work was supported in part by grant EEC9908280 from NSF (to C. Rensing and I.L. Pepper) and by funding from the Queen’s University Environmental Science and Technology Research Centre (D.J. Fairley and M.J. Larkin).

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • D. J. Fairley
    • 1
  • G. Wang
    • 4
  • C. Rensing
    • 3
  • I. L. Pepper
    • 3
  • M. J. Larkin
    • 1
    • 2
    Email author
  1. 1.Queen’s University Environmental Science & Technology Research CentreThe Queen’s University of BelfastBelfastUK
  2. 2.School of Biological SciencesThe Queen’s University of BelfastBelfastUK
  3. 3.Department of Soil, Water and Environmental ScienceThe University of ArizonaTucsonUSA
  4. 4.College of Life Sciences and TechnologyHuazhong Agricultural UniversityWuhanChina

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