Applied Microbiology and Biotechnology

, Volume 73, Issue 3, pp 533–540

Optimization of multienzyme production by two mixed strains in solid-state fermentation

Biotechnological Products and Process Engineering


F3 and F4 strains of Aspergillus niger were screened from five strains of fungi to produce multienzyme preparations (containing cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase) as dietary supplementation. Enzyme activities indicated that 1:4 (F3 to F4) was the optimum mixture proportion, and 0.3% (W/W) was the preferable pitching rate. In bran mash containing 54.5% (W/W) water, F3 and F4 could produce the supplementation better when cultured 30 to 36 h at 30 °C. Monofactorial and orthogonal experiments were performed to optimize media. Results of the variance and range analysis showed that the optimum medium contained 80 g of bran, 20 g of cottonseed powder, 1 g of (NH4)2SO4, and 0.1 g of KH2PO4. When F3 and F4 strains were cultured in the optimum medium containing 54.5% (W/W) water, the activity of cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase reached 996; 15,863; 13,378; 7,621; and 5,583 U/g, respectively.


Multienzyme supplementation Aspergillus niger Mixed strains Solid state fermentation Optimization Medium 


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.The Key Laboratory of Crop Biology of Shandong, College of Life ScienceShandong Agricultural UniversityTai’anPeople’s Republic of China
  2. 2.Fermentation Engineering Laboratory, College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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