The Fourier transform infrared (FTIR) spectra of the cells of two photosynthetic H2-producing strains, Rhodoblastus acidophilus and Rhodobacter capsulatus, as well as their extracellular polymeric substances (EPS), were evaluated. The FTIR spectra of R.capsulatus and its EPS during its cultivation were also recorded. The main peaks in the spectra, including 1,080 cm−1 (carbohydrates), 1,250 cm−1 (nucleic acids), 2,830–2,930 cm−1 (lipids), 1,660–1,535 cm−1 (Amide I and II of proteins), were observed. The relative heights of these peaks in the spectra of the two strains were different, showing the difference in contents of various components in the cells or EPS. The ratios among the main components in the EPS obtained from the FTIR spectra were in good agreement with those from a conventional quantitative chemical analysis. As an easy, rapid, and direct technique, the FTIR spectroscopy could be used to characterize the components and their relative contents of EPS of photosynthetic bacteria.
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The authors wish to thank the Natural Science Foundation (NSFC) of China (Grant No. 20577048), the NSFC-RGC Joint Project (50418009), and the Trans-Century Training Program Foundation for the Talents, Ministry of Education, China, for the partial support of this study.
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Sheng, G., Yu, H. & Wang, C. FTIR-spectral analysis of two photosynthetic H2-producing strains and their extracellular polymeric substances. Appl Microbiol Biotechnol 73, 204–210 (2006). https://doi.org/10.1007/s00253-006-0442-2
- Extracellular Polymeric Substance
- Rhodobacter Capsulatus
- Extracellular Polymeric Substance Extraction
- Typical Absorption Band
- Flocculation Ability