A low-cost medium for mannitol production by Lactobacillus intermedius NRRL B-3693
- 237 Downloads
The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic nitrogen sources and corn steep liquor were evaluated for their potential to replace more expensive nitrogen sources derived from Bacto-peptone and yeast extract. Soy peptone D (5 g/l) and corn steep liquor (50 g/l) were found to be suitable substitutes for Bacto-peptone (5 g/l) and Bacto-yeast extract (5 g/l), respectively. The bacterium produced 105 g mannitol per liter from the molasses and fructose syrup (1:1, total sugars 150 g/l; fructose:glucose 4:1) in 22 h using a combination of soy peptone D (5 g/l) and corn steep liquor (50 g/l). This is the first report on the production of mannitol by fermentation using molasses and corn steep liquor.
KeywordsFermentation Fructose Mannitol Lactic Acid Bacterium Corn Steep Liquor
The author thanks Gregory J. Kennedy for excellent technical assistance.
- De Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of Lactobacilli. J Appl Bacteriol 23:130–135Google Scholar
- Johnson JC (1976) Sugar alcohols and derivatives. In: Specialized sugars for the food industry. Noyes Data, New Jersey, p 313Google Scholar
- Korakli M, Schwarz E, Wolf G, Hammes WP (2000) Production of mannitol by Lactobacillus sanfranciscensis. Adv Food Sci 22:1–4Google Scholar
- Makkee M, Kieboom APG, Van Bekkum H (1985) Production methods of d-mannitol. Starch/Stärke 37:136–141Google Scholar
- Richter H, Hamann I, Unden G (2003) Use of the mannitol pathway in fructose fermentation of Oenococcus oeni due to limiting redox regeneration capacity of the ethanol pathway. Arch Microbiol 179:227–233Google Scholar
- Saha BC (2003) Production of mannitol by fermentation. In: Saha BC (ed) Fermentation biotechnology. American Chemical Society, Washington, District of Columbia, pp 67–85Google Scholar
- Soetaert W, Buchholz K, Vandamme EJ (1995) Production of d-mannitol and d-lactic acid by fermentation with Leuconostoc mesenteroides. Agro Food Ind Hi Technol 6:41–44Google Scholar