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Applied Microbiology and Biotechnology

, Volume 72, Issue 5, pp 982–985 | Cite as

Amplification of 1-deoxy-d-xyluose 5-phosphate (DXP) synthase level increases coenzyme Q10 production in recombinant Escherichia coli

  • Soo-Jung Kim
  • Myoung-Dong Kim
  • Jin-Ho Choi
  • Sang-Yong Kim
  • Yeon-Woo Ryu
  • Jin-Ho SeoEmail author
Applied Genetics and Molecular Biotechnology

Abstract

For the enhancement of coenzyme Q10 (CoQ10) production, 1-deoxy-d-xylulose 5-phosphate (DXP) synthase of Pseudomonas aeruginosa was constitutively coexpressed in a recombinant Escherichia coli strain, which harbors the ddsA gene from Gluconobacter suboxydans encoding decaprenyl diphosphate synthase. It was found that the expression of the ddsA gene caused depletion of the isopentenyl diphosphate (IPP) pool in E. coli. Amplification of DXP synthase level by installing P. aeruginosa DXP synthase restored the diminished IPP pool and concomitantly resulted in approximately a twofold increase in relative content and productivity of CoQ10. Maximum CoQ10 concentration of 46.1 mg l−1 was achieved from glucose-limited fed-batch cultivation of the recombinant E. coli strain simultaneously harboring the ddsA and dxs genes.

Keywords

Lycopene CoQ10 Isopentenyl Diphosphate CoQ10 Production CoQ10 Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by Korea Ministry of Commerce, Industry and Trade, and Ministry of Education through the BK21 program and in part by Korea Research Foundation Grant funded by Korean government (KRF-2004-005-F00055).

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Soo-Jung Kim
    • 1
  • Myoung-Dong Kim
    • 4
  • Jin-Ho Choi
    • 1
  • Sang-Yong Kim
    • 2
  • Yeon-Woo Ryu
    • 3
  • Jin-Ho Seo
    • 1
    Email author
  1. 1.Department of Agricultural Biotechnology and Research Center for Agricultural BiomaterialsSeoul National UniversitySeoulSouth Korea
  2. 2.BioNgene Co., Ltd.SeoulSouth Korea
  3. 3.Department of Molecular Science and TechnologyAjou UniversitySuwonSouth Korea
  4. 4.School of Biotechnology and BioengineeringKangwon National UniversityChuncheonSouth Korea

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