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Direct electrochemical immunoassay based on a silica nanoparticles/sol–gel composite architecture for encapsulation of immunoconjugate

Abstract

A highly hydrophobic and non-toxic colloidal silica nanoparticle/polyvinyl butyral sol–gel composite membrane was prepared on a platinum wire electrode. With diphtheria-toxoid (D-Ag) as a model antigen and encapsulation of diphtheria antibody (D-Ab) in the composite architecture, this membrane could be used for reagentless electrochemical immunoassay. It displayed a porous and homogeneous composite architecture without the aggregation of the immobilized protein molecules. The formation of immunoconjugate by a simple one-step immunoreaction between D-Ag in sample solution and the immobilized D-Ab introduced the change in the potential. Under optimal conditions, the D-Ag analyte could be determined in the linear ranges from 10 to 800 ng ml−1 with a relatively low detection limit of 2.3 ng ml−1 at 3δ. The D-Ag immunosensor exhibited good precision, high sensitivity, acceptable stability, accuracy, and reproducibility. This composite membrane could be used efficiently for the entrapment of different biomarkers and clinical applications.

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References

  1. Aassi MZ, Van-Oss CJ, Absolom, DR (eds) (1984) Molecular immunology. Marcel Dekker, New York

  2. Chen RT, Broome CV, Weinstein RA, Weaver R, Tsa TF (1985) Diphtheria in the United States, 1971–1981. Am J Publ Health 75:1365–1393

  3. Hianik T, Snejdarkova M, Sokolikova L, Meszar E, Krivanek R, Tvarozek V, Novotny I, Wang J (1999) Immunosensors based on supported lipid membranes, protein films and liposomes modified by antibodies. Sens Actuators B 57:201–212

  4. Katz E, Willner I (2003) Probing biomolecular interactions at conductive and semiconductive surfaces by impedance spectroscopy: routes to impedance immunosensors, DNA-sensors, and enzyme biosensors. Electroanalysis 15:913–947

  5. Liu GD, Wu ZY, Wang SP, Shen GL, Yu RQ (2001) Renewable amperometric immunosensor for Schistosoma japonicum antibody assay. Anal Chem 73:3219–3226

  6. Liu YJ, Yin F, Long YM, Zhang ZH, Yao SZ (2003) Study of the immobilization of alcohol dehydrogenase on Au-colloid modified gold electrode by piezoelectric quartz crystal sensor, cyclic voltammetry, and electrochemical impedance techniques. J Colloi Interface Sci 258:75–81

  7. Nikolelis DP, Mitrokotsa M (2004) Rapid electrochemical detection of propranolol and metoprolol in pharmaceutical preparations using stabilized lipid films. Langmuir 16:741–747

  8. Storri S, Santoni T, Minunni M, Mascini M (1998) Surface modifications for the development of piezoimmunosensors. Biosens Bioelectron 13:347–357

  9. Tang FQ, Li JR, Zhang L, Jiang L (1992) Improvement of enzymatic activity and lifetime of Langmuir–Blodgett films by using submicron SiO2 particles. Biosens Bioelectron 7:503–507

  10. Tang FQ, Men XW, Chen D, Ran JG, Gou L, Zhen CQ (2000) Nanoparticles-enhance glucose biosensor. Sci China B 30:119–124

  11. Tang D, Yuan R, Chai Y, Fu Y, Dai J, Liu Y, Zhong X (2005) New amperometric and potentiometric immunosensors based on gold nanoparticles/tris(2,2′-bipyridyl) cobalt(III) multilayer films for hepatitis B surface antigen determinations. Biosens Bioelectron 21:539–548

  12. Wang H, Wu ZY, Shen GL, Yu RQ (2001) A novel piezoelectric immunosensor for the determination of transferrin. Chem J Chin Univ 22:1305–1309

  13. Wang CC, Wang H, Wu ZY, Shen GL, Yu RQ (2002) A piezoelectric immunoassay based on self-assembled monolayers of cystamine and polystyrene sulfonate for determination of Schistosoma japonicum antibodies. Anal Bioanal Chem 373:803–809

  14. Wharton M, Vitek CR (2003) Diphtheria. In: Plotkin SA, Orenstein WA (eds) Vaccines, 4th edn. Saunders, Philadelphia, PA, p 211

  15. Wu Z, Li J, Luo M, Shen G, Yu R (2005) A novel capacitive immunosensor based on gold colloid monolayers associated with a sol–gel matrix. Anal Chim Acta 528:235–242

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Acknowledgements

This work was supported by the NNSF of China (29705001), the Chinese Education Ministry Foundation for Excellent Young Teachers (No. 2002-40) and the NSF of Chongqing City (No. 20027477), China.

Author information

Correspondence to Ruo Yuan.

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Wang, F., Yuan, R. & Chai, Y. Direct electrochemical immunoassay based on a silica nanoparticles/sol–gel composite architecture for encapsulation of immunoconjugate. Appl Microbiol Biotechnol 72, 671–675 (2006). https://doi.org/10.1007/s00253-006-0353-2

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Keywords

  • Silica Nanoparticles
  • Composite Membrane
  • Diphtheria
  • Potentiometric Response
  • Polyvinyl Butyral