Bacterial stress enrichment enhances anaerobic hydrogen production in cattle manure sludge

  • Dae-Yeol Cheong
  • Conly L. HansenEmail author
Biotechnological Products and Process Engineering


Methodology was evaluated to selectively enrich hydrogen-producing species present in biological sludge produced during organic wastewater treatment. The influence of bacterial stress enrichment on anaerobic hydrogen-producing microorganisms was investigated in batch tests using serum bottles. Enrichment conditions investigated included application of acute physical and chemical stresses: wet heat, dry heat and desiccation, use of a methanogen inhibitor, freezing and thawing, and chemical acidification with and without preacidification of the sludge at pH 3. For each enrichment sample, cultivation pH value was set at an initial value of 7. After application of selective enrichment (by bacterial stress), hydrogen production was significantly higher than that of untreated original sludge. Hydrogen production from the inocula with bacterial stress enrichment was 1.9–9.8 times greater when compared with control sludge. Chemical acidification using perchloric acid showed the best hydrogen production potential, irrespective of preacidification. Enhancement is due to the selective capture of hydrogen-producing sporeformers, which induces altered anaerobic fermentative metabolism.


Sludge Hydrogen Production Volatile Suspended Solid Total Kjeldahl Nitrogen Anaerobic Fermentation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported in part by the Utah Agricultural Experiment Station, Utah State University, Logan, Utah 84322-4810, JP No. 7682.


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© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Department of Biological and Irrigation EngineeringUtah State UniversityLoganUSA

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