Advertisement

Bacterial stress enrichment enhances anaerobic hydrogen production in cattle manure sludge

  • Dae-Yeol Cheong
  • Conly L. HansenEmail author
Biotechnological Products and Process Engineering

Abstract

Methodology was evaluated to selectively enrich hydrogen-producing species present in biological sludge produced during organic wastewater treatment. The influence of bacterial stress enrichment on anaerobic hydrogen-producing microorganisms was investigated in batch tests using serum bottles. Enrichment conditions investigated included application of acute physical and chemical stresses: wet heat, dry heat and desiccation, use of a methanogen inhibitor, freezing and thawing, and chemical acidification with and without preacidification of the sludge at pH 3. For each enrichment sample, cultivation pH value was set at an initial value of 7. After application of selective enrichment (by bacterial stress), hydrogen production was significantly higher than that of untreated original sludge. Hydrogen production from the inocula with bacterial stress enrichment was 1.9–9.8 times greater when compared with control sludge. Chemical acidification using perchloric acid showed the best hydrogen production potential, irrespective of preacidification. Enhancement is due to the selective capture of hydrogen-producing sporeformers, which induces altered anaerobic fermentative metabolism.

Keywords

Sludge Hydrogen Production Volatile Suspended Solid Total Kjeldahl Nitrogen Anaerobic Fermentation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This research was supported in part by the Utah Agricultural Experiment Station, Utah State University, Logan, Utah 84322-4810, JP No. 7682.

References

  1. APHA (1992) Standard methods for the examination of water and wastewater, 18th edn. American Public Health Association, Washington, DCGoogle Scholar
  2. Bahl H, Gottschalk G (1984) Parameters affecting solvent production by Clostridium acetobutyricum in continuous culture. Biotechnol Bioeng Symp 14:215–223Google Scholar
  3. Benemann JR (1996) Hydrogen biotechnology: progress and prospects. Nat Biotechnol 14:1101–1103CrossRefPubMedGoogle Scholar
  4. Benemann JR (1998) The technology of biohydrogen. In: Zaborsky OR (ed) Biohydrogen. Plenum, New York pp 19−30Google Scholar
  5. Billings RE (1991) The hydrogen world view. American Academy of Science, Washington, DCGoogle Scholar
  6. Brock TD, Madigan MT, Martinko JM, Parker J (1994) Biology of microorganisms. Prentice-Hall, New YorkGoogle Scholar
  7. Chen CC, Lin CY, Chang JS (2001) Kinetics of hydrogen production with continuous anaerobic cultures utilizing sucrose as the limiting substrate. Appl Microbiol Biotechnol 57:56–64CrossRefPubMedGoogle Scholar
  8. Chen CC, Lin CY, Lin MC (2002) Acid–base enrichment enhances anaerobic hydrogen production process. Appl Microbiol Biotechnol 58:224–228CrossRefPubMedGoogle Scholar
  9. Chin HL, Chen ZS, Chou CP (2003) Fedbatch operation using Clostridium acetobutyricum suspension culture as biocatalyst for enhancing H2 production. Biotechnol Prog 19:383–388CrossRefPubMedGoogle Scholar
  10. Das D, Veziroglu TN (2001) Hydrogen production by biological processes: a survey of literature. Int J Hydrogen Energy 26:13–28CrossRefGoogle Scholar
  11. Dunn S (2002) Hydrogen futures: toward a sustainable energy system. Int J Hydrogen Energy 27:235–264CrossRefGoogle Scholar
  12. Fang HHP, Zhang T, Liu H (2002) Microbial diversity of a mesophilic hydrogen-producing sludge. Appl Microbiol Biotechnol 58:112–118CrossRefPubMedGoogle Scholar
  13. Gottschalk G (1986) Bacterial metabolism. Springer, Berlin Heidelberg New YorkGoogle Scholar
  14. Harper SR, Pohland FG (1986) Recent developments in hydrogen management during anaerobic biological wastewater treatment. Biotechnol Bioeng 28:585–602CrossRefGoogle Scholar
  15. Hart D (1997) Hydrogen power: the commercial future of the ultimate fuel. Financial Times Energy Publishing, LondonGoogle Scholar
  16. Henley M, Wallace W, Andrel T (2003) Microbial hydrogen production. AFRL Technol Horiz 3:32–33Google Scholar
  17. Heyndrickx M, De Vos P, Stevens T, De Ley J (1987) Effect of various external factors on the factors on the fermentative production of hydrogen gas from glucose by Clostridium butyricum strains in batch culture. Syst Appl Microbiol 9:163–168Google Scholar
  18. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams and Wilkins, Baltimore, MDGoogle Scholar
  19. Hyun HH, Zeikus JG, Longin R, Millet J, Ryter A (1983) Ultrastructure and extreme heat resistance of spores from thermophilic Clostridium species. J Bacteriol 156:1332–1337PubMedGoogle Scholar
  20. Ince BK, Ince O (2000) Changes to bacterial community make-up in a two-phase anaerobic digestion system. J Chem Technol Biotechnol 75:500–508CrossRefGoogle Scholar
  21. Jean DS, Chang BV, Liao GS, Tsou GW, Lee DJ (2000) Reduction of microbial density level in sewage through pH adjustment and ultrasonic treatment. Water Sci Technol 42:97–102Google Scholar
  22. Jungermann KA, Thauer RK, Leimenstoll G, Deker K (1973) Function of reduced pyridine nucleotide–ferredoxin oxidoreductases in saccharolytic clostridia. Biochim Biophys Acta 305:268–280PubMedCrossRefGoogle Scholar
  23. Karube I, Urano N, Matsunaga T, Suzuki S (1982) H2 production from glucose by immobilized growing cells of Clostridium butyricum. Eur J Appl Microbiol 16:5–9CrossRefGoogle Scholar
  24. Kataoka N, Miya A, Kiriyama K (1997) Studies on hydrogen production by continuous culture system of hydrogen-producing anaerobic bacteria. Water Sci Technol 36:41–47CrossRefGoogle Scholar
  25. Kenealy WR, Cao Y, Weimer PJ (1995) Production of caproic acid by cocultures of ruminal cellulolytic bacteria and Clostridium kluveri grown on cellulose and ethanol. Appl Microbiol Biotechnol 44:507–513CrossRefPubMedGoogle Scholar
  26. Kim BH, Bellows P, Datta R, Zeikus JG (1984) Control of carbon and electron flow in Clostridium acetobutyricum fermentation: utilization of carbon monoxide to inhibit hydrogen production and to enhance butanol yields. Appl Environ Microbiol 48:764–770PubMedGoogle Scholar
  27. Kumar N, Das D (2000) Enhancement of hydrogen production by Enterobacter cloacae IIT-BT 08. Proc Biochem 35:589–593CrossRefGoogle Scholar
  28. Kunst A, Draeger B, Ziegenhorn J (1983) UV-methods with hexokinase and glucose-6-phosphate dehydrogenase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, 3rd edn. Metabolites 1: carbohydrates, vol 6. Chemie, Weinheim, pp 163−172Google Scholar
  29. Lamed RJ, Lobos JH, Su TM (1988) Effect of stirring and hydrogen on fermentation products of Clostridium thermocellum. Appl Environ Microbiol 54:1216–1220PubMedGoogle Scholar
  30. Lay JJ (2001) Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose. Biotechnol Bioeng 74:280–286CrossRefPubMedGoogle Scholar
  31. Lay JJ, Li YY, Noike T (1998) A mathematical model for methane production from a landfill bioreactor. J Environ Eng ASCE 124:730–736CrossRefGoogle Scholar
  32. Lay JJ, Lee YJ, Noike T (1999) Feasibility of biological hydrogen production from organic fraction of municipal solid waste. Water Res 33:2579–2586CrossRefGoogle Scholar
  33. Lee YJ, Miyahara T, Noike T (2002) Effect of pH on microbial hydrogen fermentation. J Chem Technol Biotechnol 77:694–698CrossRefGoogle Scholar
  34. Levin D, Pitt L, Love M (2004) Biohydrogen production: prospectus and limitations to practical application. Int J Hydrogen Energy 29:173–185CrossRefGoogle Scholar
  35. Liu CL, Peck HD (1981) Comparative bioenergetics of sulfate reduction in Desulfovibrio and Desulfotomaculum sp. J Bacteriol 145:966–973PubMedGoogle Scholar
  36. Lupton FS, Conrad R, Zeikus JG (1984) Physiological function of hydrogen metabolism during growth of sulfidogenic bacteria on organic substrates. J Bacteriol 159:843–849PubMedGoogle Scholar
  37. Minton NP, Clarke DJ (1989) Clostridia—biotechnology handbook, vol 3. Plenum, New YorkGoogle Scholar
  38. Mizuno O, Dinsdale R, Hawkes FR, Hawkes DL, Noike T (2000) Enhancement of hydrogen production from glucose by nitrogen gas sparging. Bioresour Technol 73:59–65CrossRefGoogle Scholar
  39. Nandi S, Sengupta S (1998) Microbial production of hydrogen: an overview. Crit Rev Microbiol 24:61–84CrossRefPubMedGoogle Scholar
  40. Oehlert GW (2000) A first course in design and analysis of experiments. Freeman, New YorkGoogle Scholar
  41. Ohwaki K, Hungate RE (1977) Hydrogen utilization by clostridia in sewage sludge. Appl Environ Microbiol 33:1270–1274PubMedGoogle Scholar
  42. Okamoto M, Miyahara T, Mizuno O, Noike T (2000) Biological hydrogen potential of materials characteristic of the organic fraction of municipal solid wastes. Water Sci Technol 41:25–32PubMedGoogle Scholar
  43. Olson JC, Nottingham PM (1980) Temperature. In: Silliker JH, Elliott RP, Baird-Parker AC, Bryan FL, Christian JHB, Clark DS, Olson JC, Roberts TA (eds) Microbial ecology of foods: factors affecting life and death of microorganisms. Academic, New York pp 1–37Google Scholar
  44. Postgate JR (1965) Recent advances in the study of the sulfate-reducing bacteria. Bacteriol Rev 29:425–441PubMedGoogle Scholar
  45. Reimann A, Biebl H, Deckwer WD (1996) Influence of iron, phosphate and methyl viologen on glycerol fermentation of Clostridium butyricum. Appl Microbiol Biotechnol 45:47–50CrossRefGoogle Scholar
  46. Setlow P (2000) Resistance of bacterial spores. In: Storz G, Hengge-Aronis R (eds) Bacterial stress responses. ASM Press, Washington, DC, pp 217–230Google Scholar
  47. Sonenshein (2000) Bacterial sporulation: a response to environmental signals. In: Storz G, Hengge-Aronis R (eds) Bacterial stress responses. ASM Press, Washington, DC pp 199–215Google Scholar
  48. Sparling R, Risbey D, Poggi-Varaldo HM (1997) Hydrogen production from inhibited anaerobic composters. Int J Hydrogen Energy 22:563–566CrossRefGoogle Scholar
  49. Speece RE (1996) Anaerobic biotechnology for industrial wastewaters. Archae Press, Nashville, TNGoogle Scholar
  50. Suzuki S, Karube I (1983) Energy production with immobilized cells. Appl Biochem Bioeng 4:281–310Google Scholar
  51. Sylvia DM, Fuhrmann JJ, Hartel PG, Zuberer DA (1999) Principles and applications of soil microbiology. Prentice-Hall, Englewood Cliffs, NJGoogle Scholar
  52. Taguchi F, Mizukami N, Taki TS, Hasegawa K (1995) Hydrogen production from continuous fermentation of xylose during growth of Clostridium sp. strain no-2. Can J Microbiol 41:536–540CrossRefGoogle Scholar
  53. Takabatake H, Suzuki K, Ko I-B, Noike T (2004) Characteristics of anaerobic ammonia removal by a mixed culture of hydrogen producing photosynthetic bacteria. Bioresour Technol 95:151–158CrossRefPubMedGoogle Scholar
  54. Tanisho S, Kamiya N, Wako N (1989) Hydrogen evolution of Enterobacter aerogens depending on culture pH: mechanism of hydrogen evolution from NADH by means of membrane-bound hydrogenase. Biochim Biophys Acta 973:1–6PubMedGoogle Scholar
  55. Thauer RK, Jungermann KA, Decker K (1977) Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev 41:100–180PubMedGoogle Scholar
  56. Ueno Y, Haruta S, Ishii M, Igarashi Y (2001) Microbial community in anaerobic hydrogen-producing microflora enriched from sludge compost. Appl Microbiol Biotechnol 57:555–562CrossRefPubMedGoogle Scholar
  57. Van Ginkel S, Sung S, Lay JJ (2001) Biohydrogen production as a function of pH and substrate concentration. Environ Sci Technol 35:4726–4730CrossRefPubMedGoogle Scholar
  58. Walther R, Hippe H, Gottschalk G (1977) Citrate, a specific substrate for the isolation of Clostridium sphenoides. Appl Environ Microbiol 33:955–962PubMedGoogle Scholar
  59. Wang CC, Chang CW, Chu CP, Lee DJ, Chang BV (2003a) Sequential production of hydrogen and methane from wastewater sludge using anaerobic fermentation. J Chin Inst Chem Eng 34:683–687Google Scholar
  60. Wang CC, Chang CW, Chu CP, Lee DJ, Chang BV, Liao CS (2003b) Producing hydrogen from wastewater sludge by Clostridium bifermentans. J Biotechnol 102:83–92CrossRefPubMedGoogle Scholar
  61. Zajic JE, Kosaric N, Brosseau JD (1978) Microbial production of hydrogen. Adv Biochem Eng/Biotechnol 7:57–109CrossRefGoogle Scholar
  62. Zehnder AJB (1988) Biology of anaerobic microorganism. Wiley, New YorkGoogle Scholar
  63. Zehnder AJB, Huser BA, Brock TD, Wuhrmann K (1980) Characterization of an acetate decarboxylating, non-hydrogen-oxidizing methane bacterium. Arch Microbiol 124:1–11CrossRefPubMedGoogle Scholar
  64. Zinder SH, Anguish T, Cardwell SC (1984) Selective inhibition by 2-bromoethanesulfonate of methanogenesis from acetate in a thermophilic anaerobic digester. Appl Environ Microbiol 47:1343–1345PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Department of Biological and Irrigation EngineeringUtah State UniversityLoganUSA

Personalised recommendations