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Applied Microbiology and Biotechnology

, Volume 69, Issue 4, pp 456–462 | Cite as

Modeling of growth kinetics for Pseudomonas spp. during benzene degradation

  • D.-J. Kim
  • J.-W. Choi
  • N.-C. Choi
  • B. Mahendran
  • C.-E. Lee
Applied Microbial and Cell Physiology

Abstract

A modeling study was conducted on growth kinetics of three different strains of Pseudomonas spp. (Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida) during benzene degradation to determine optimum substrate concentrations for most efficient biodegradation. Batch tests were performed for eight different initial substrate concentrations to observe cell growth and associated substrate degradation using benzene-adapted cells. Kinetic parameters of both inhibitory (Haldane–Andrews, Aiba–Edwards) and noninhibitory (Monod) models were fitted to the relationship between specific growth rate and substrate concentration obtained from the growth curves. Results showed that half-saturation constant of P. fluorescens was the highest among the three strains, indicating that this strain could grow well at high concentration, while P. putida could grow best at low concentration. The inhibition constant of P. aeruginosa was the highest, implying that it could tolerate high benzene concentration and therefore could grow at a wider concentration range. Estimated specific growth rate of P. putida was lower, but half-saturation constant was higher than those from literature study due to high substrate concentration range used in this study. These two kinetic parameters resulted in substantial difference between Monod- and Haldane-type models, indicating that distinction should be made in applying those models.

Keywords

Substrate Concentration Specific Growth Rate Haldane Mineral Salt Medium Substrate Degradation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by the Korea Research Foundation (grant no. KRF-2004-005-C00060).

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • D.-J. Kim
    • 1
  • J.-W. Choi
    • 1
  • N.-C. Choi
    • 1
  • B. Mahendran
    • 1
  • C.-E. Lee
    • 2
  1. 1.Department of Earth and Environmental SciencesKorea UniversitySeoulSouth Korea
  2. 2.Institute for Nano ScienceKorea UniversitySeoulSouth Korea

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