Trichloroethylene degradation by butane-oxidizing bacteria causes a spectrum of toxic effects
- 212 Downloads
The physiological consequences of trichloroethylene (TCE) transformation by three butane oxidizers were examined. Pseudomonas butanovora, Mycobacterium vaccae, and Nocardioides sp. CF8 utilize distinctly different butane monooxygenases (BMOs) to initiate degradation of the recalcitrant TCE molecule. Although the primary toxic event resulting from TCE cometabolism by these three strains was loss of BMO activity, species differences were observed. P. butanovora and Nocardioides sp. CF8 maintained only 4% residual BMO activity following exposure to 165 μM TCE for 90 min and 180 min, respectively. In contrast, M. vaccae maintained 34% residual activity even after exposure to 165 μM TCE for 300 min. Culture viability was reduced 83% in P. butanovora, but was unaffected in the other two species. Transformation of 530 nmol of TCE by P. butanovora (1.0 mg total protein) did not affect the viability of BMO-deficient P. butanovora cells, whereas transformation of 482 nmol of TCE by toluene-grown Burkholderia cepacia G4 caused 87% of BMO-deficient P. butanovora cells to lose viability. Together, these results contrast with those previously reported for other bacteria carrying out TCE cometabolism and demonstrate the range of cellular toxicities associated with TCE cometabolism.
KeywordsSodium Butyrate Nocardioides Nitrosomonas Europaea Methylosinus Trichosporium Primary Toxic Event
This work was supported by the office of Research and Development, United States Environmental Protection Agency, under Agreement R-828772 through the Western Region Hazardous Substance Research Center.
- Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the Biuret reaction. J Biol Chem 177:751–766Google Scholar
- Hamamura N, Page C, Long T, Semprini L, Arp DJ (1997) Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and methane-grown Methylosinus trichosporium OB3b. Appl Environ Microbiol 63:3607–3613Google Scholar
- Kim Y, Semprini L, Arp DJ (1997) Aerobic cometabolism of chloroform, 1,1,1-trichloroethane, and other chlorinated aliphatic hydrocarbons by butane-utilizing microorganisms. In: Alleman BC, Lecson A (eds) In-Situ and On-Site Bioremediation 4(3). Battelle Press, Columbus, CH, pp 247–252Google Scholar
- Silverman RB (1988) Mechanism-based inactivation: chemistry and enzymology. CRC, Boca RatonGoogle Scholar
- Hylckama Vlieg JET van, Koning W de, Janssen DB (1996) Transformation kinetics of chlorinated ethenes by Methylosinus trichosporium OB3b and detection of unstable epoxides by on-line gas chromatography. Appl Environ Microbiol 62:3304–3312Google Scholar
- Hylckama Vlieg JET van, Koning W de, Janssen LP (1997) Effect of chlorinated ethene conversion on viability and activity of Methylosinus trichosporium OB3b. Appl Environ Microbiol 63:4961–4964Google Scholar
- Wiegant WW, Bont JAM de (1980) A new route for ethylene glycol metabolism in Mycobacterium E44. J Gen Microbiol 120:325–331Google Scholar