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Influence of temperature on process efficiency and microbial community response during the biological removal of chlorophenols in a packed-bed bioreactor

Abstract

Two reactors, initially operated at 14 and 23±1°C (RA and RB, respectively), were inoculated with a bacterial consortium enriched and acclimatized to the respective temperatures over 4 months. The biofilms, formed in the reactors, were studied using scanning electron microscopy, cultivation of the biofilm microflora, and physiological analysis of the isolates. Two bacteria able to mineralize chlorophenols under a large range of temperature (10–30°C) were isolated from the biofilm communities of reactors RA and RB and characterized as Alcaligenaceae bacterium R14C4 and Cupriavidus basilensis R25C6, respectively. When temperature was decreased by 10°C, the chlorophenols removal capacity was reduced from 51.6 to 22.8 mg l−1 h−1 in bioreactor RA (from 14 to 4°C) and from 59.3 to 34.7 mg l−1 h−1 in bioreactor RB (from 23±1 to 14°C). Fluorescence in situ hybridization (FISH) of the biofilm communities showed that, in all temperatures tested, the β-proteobacteria were the major bacterial community (35–47%) followed by the γ-proteobacteria (12.0–6.5%). When the temperature was decreased by 10°C, the proportions of γ-proteobacteria and Pseudomonas species increased significantly in both microbial communities.

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Acknowledgements

This research was funded by SIDA (the Swedish International Development Cooperation Agency). The ministry of science, research, and technology of the Islamic Republic of Iran provided financial support to Hamid Zilouei. The Portuguese Fundacao para a Ciencia e a Tecnologia is also acknowledged for supporting Ana Soares through grant BD/13083/2003.

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Correspondence to B. Guieysse.

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Zilouei, H., Soares, A., Murto, M. et al. Influence of temperature on process efficiency and microbial community response during the biological removal of chlorophenols in a packed-bed bioreactor. Appl Microbiol Biotechnol 72, 591–599 (2006). https://doi.org/10.1007/s00253-005-0296-z

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Keywords

  • Internal Transcribe Spacer
  • Hydraulic Retention Time
  • Chlorophenols
  • Mineral Salt Medium
  • Achromobacter Xylosoxidans