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Applied Microbiology and Biotechnology

, Volume 71, Issue 4, pp 440–443 | Cite as

An aqueous–organic two-phase bioprocess for efficient production of the natural aroma chemicals 2-phenylethanol and 2-phenylethylacetate with yeast

  • M. M. W. Etschmann
  • J. SchraderEmail author
Biotechnological Products and Process Engineering

Abstract

The natural aroma chemicals 2-phenylethanol (2-PE) and 2-phenylethylacetate (2-PEAc) are of high industrial relevance and can be produced from l-phenylalanine in a yeast-based process with growth-associated product formation. Due to product inhibition, in situ product removal is mandatory to obtain economically interesting concentrations. A fed-batch approach using polypropylene glycol 1200 as in situ extractant and the precursor in a saturated concentration led to the highest 2-PE productivity reported for a bioprocess so far. With Kluyveromyces marxianus CBS 600, 26.5 g/l 2-PE and 6.1 g/l 2-PEAc in the organic phase were obtained, corresponding to space–time yields of 0.33 and 0.08 g/l h, respectively.

Keywords

Time Yield Oleyl Alcohol Kluyveromyces Marxianus Polypropylene Glycol Pyridoxine Hydrochloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Dechema e.V, Karl-Winnacker-Institut, Biochemical Engineering GroupFrankfurt am MainGermany

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