Applied Microbiology and Biotechnology

, Volume 72, Issue 1, pp 163–167 | Cite as

Cellulase production by Penicillium echinulatum on lactose

  • Nicole Teixeira Sehnem
  • Luciano Ramos de Bittencourt
  • Marli Camassola
  • Aldo J. P. DillonEmail author
Applied Microbial and Cell Physiology


The inducer effect of lactose on cellulase activity in Penicillium echinulatum 9A02S1 was studied. Submerged cultivation was performed using different concentrations of lactose and cellulose, in which the pH, mycelial mass, soluble proteins, filter paper activity (FPA), and activity of β-glucosidases were measured. The cultures containing lactose only presented low FPAs (0.1 FPU/ml). The cultures with associated cellulose and lactose and those containing cellulose only presented similar enzymatic activities (1.5 FPU/ml), suggesting the possibility of up to 75% reduction in the cellulose concentration. In relation to the β-glucosidases, increasing the lactose/cellulose ratio results in a proportional increase of enzymatic activity. In the cultures using both inducers, there is a longer duration of the acid phase in relation to treatments using only cellulose or lactose, indicating diauxia and catabolic repression.


Cellulose Cellulase Lactose Cellobiose Cellulase Production 
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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Nicole Teixeira Sehnem
    • 1
  • Luciano Ramos de Bittencourt
    • 1
  • Marli Camassola
    • 1
  • Aldo J. P. Dillon
    • 1
    Email author
  1. 1.Institute of BiotechnologyUniversity of de Caxias do SulCaxias do SulBrazil

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