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Applied Microbiology and Biotechnology

, Volume 72, Issue 1, pp 155–162 | Cite as

Generation of aroma compounds from Ditaxis heterantha by Saccharomyces cerevisiae

  • L. Del Toro-Sánchez
  • S. Sánchez
  • M. A. Ortiz
  • S. Villanueva
  • E. Lugo-CervantesEmail author
Applied Microbial and Cell Physiology

Abstract

Ditaxis heterantha, a plant of the Euphorbiaceae family, is growing wild in the semiarid regions of Mexico. The seed endosperm contains yellow pigments (carotenoids). By high-pressure liquid chromatography the total pigment (TP) was separated into seven fractions: two of them, heterathin (F4) and ditaxin (F5), characterized as apocarotenoids, represent 80% of TP. Both molecules have double bonds, which seem to be the target for degradation and aroma formation. In this work, TP, F4, and F5 were supplied to nine cultures able to degrade lutein. From these strains, only one (identified as Saccharomyces cerevisiae) was able to produce aromas from either TP or F4. Using TP as substrate, the produced aromas were 4-oxo-isophorone (1), isophorone (2), cinnamic aldehyde (6), 3-hydroxy-β-cyclocitral (7), safranal (8), geranyl (9), 3-oxo-α-ionone (10), 3-oxo-α-ionol (11), 3-oxo-7,8-dihydro-α-ionone (12), and eugenol (13). Of these aromas, only seven were produced from F4: (1), (2), (7), (8), (10), (11), and (12). In both cases, safranal was the main degradation product (30%). The enzymatic activity responsible for this effect was found in the cytosolic fraction and detected only when S. cerevisiae was grown in the presence of TP or F4.

Keywords

Carotenoid Lutein Saffron Isophorone Total Pigment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This research was financially supported by the Consejo Nacional de Ciencia y tecnología (CONACYT), Simorelos program (grant 1990305012), and Consejo estatal de ciencia y Tecnología de Jalisco (COECYTJAL, grant 1811). We thank Gabriela Maldonado-Robledo, Eduardo Rodríguez-Bustamante, Mirna Estarrón-Espinoza for their technical assistance in the realization of this work and Hector Escalona-Buendia and Peter Knauth for their help with the text.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • L. Del Toro-Sánchez
    • 1
    • 2
  • S. Sánchez
    • 2
  • M. A. Ortiz
    • 2
  • S. Villanueva
    • 1
  • E. Lugo-Cervantes
    • 1
    Email author
  1. 1.Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de JaliscoGuadalajara JaliscoMexico
  2. 2.Departamento de Biología Molecular y Biotecnología del Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoMexicoMexico

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