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Applied Microbiology and Biotechnology

, Volume 72, Issue 1, pp 52–59 | Cite as

Sophorolipid produced from the new yeast strain Wickerhamiella domercqiae induces apoptosis in H7402 human liver cancer cells

  • Jing Chen
  • Xin Song
  • Hui Zhang
  • Yin-bo Qu
  • Jun-ying Miao
Biotechnological Products and Process Engineering

Abstract

The effects of sophorolipid on the growth and apoptosis of H7402 human liver cancer cells were investigated. By treatment with sophorolipid, a dose- and time-dependent inhibition of cell proliferation was observed. The cells developed many features of apoptosis, including condensation of chromatin, nuclear fragmentation, and appearance of apoptotic bodies, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling positive cells were stained dark brown. Sophorolipid treatment induced apoptosis in H7402 cells by blocking cell cycle at G1 phase and partly at S phase, activating caspase-3, and increasing Ca2+ concentration in cytoplasm. These findings may suggest a potential use of sophorolipid for liver cancer treatment.

Keywords

H7402 Cell Cell Cycle Distribution Normal Liver Cell Sophorolipids Sophorose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Jing Chen
    • 1
  • Xin Song
    • 1
  • Hui Zhang
    • 2
  • Yin-bo Qu
    • 1
  • Jun-ying Miao
    • 2
  1. 1.State Key Lab of Microbial TechnologyShandong UniversityJinanPeople’s Republic of China
  2. 2.Institute of Developmental Biology, School of Life ScienceShandong UniversityJinanChina

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