Applied Microbiology and Biotechnology

, Volume 72, Issue 1, pp 52–59 | Cite as

Sophorolipid produced from the new yeast strain Wickerhamiella domercqiae induces apoptosis in H7402 human liver cancer cells

  • Jing Chen
  • Xin Song
  • Hui Zhang
  • Yin-bo Qu
  • Jun-ying Miao
Biotechnological Products and Process Engineering


The effects of sophorolipid on the growth and apoptosis of H7402 human liver cancer cells were investigated. By treatment with sophorolipid, a dose- and time-dependent inhibition of cell proliferation was observed. The cells developed many features of apoptosis, including condensation of chromatin, nuclear fragmentation, and appearance of apoptotic bodies, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling positive cells were stained dark brown. Sophorolipid treatment induced apoptosis in H7402 cells by blocking cell cycle at G1 phase and partly at S phase, activating caspase-3, and increasing Ca2+ concentration in cytoplasm. These findings may suggest a potential use of sophorolipid for liver cancer treatment.


H7402 Cell Cell Cycle Distribution Normal Liver Cell Sophorolipids Sophorose 
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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Jing Chen
    • 1
  • Xin Song
    • 1
  • Hui Zhang
    • 2
  • Yin-bo Qu
    • 1
  • Jun-ying Miao
    • 2
  1. 1.State Key Lab of Microbial TechnologyShandong UniversityJinanPeople’s Republic of China
  2. 2.Institute of Developmental Biology, School of Life ScienceShandong UniversityJinanChina

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