Applied Microbiology and Biotechnology

, Volume 71, Issue 6, pp 870–874 | Cite as

Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: a potential tool in metabolic engineering

  • Henry Lin
  • Natalia M. Castro
  • George N. Bennett
  • Ka-Yiu San
Applied Genetics and Molecular Biotechnology


The overexpression of acetyl-CoA (CoA) synthetase (ACS) in Escherichia coli showed significant reduction in acetate during glucose fermentation. It also greatly enhanced acetate assimilation when acetate was used as a carbon source. These features are ideal for applications in metabolic engineering. ACS overexpression can be strategically applied to reduce acetate byproduct, recover wasted carbon, and redirect carbon flux toward more favorable pathways. The native acs gene was cloned and overexpressed in E. coli. Studies showed significant effects on acetate production and assimilation in cultures grown in minimal and complex media with glucose or acetate as the carbon source.


Fermentation Acetate Production Isoamyl Acetate Succinate Production Strain MG1655 
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This work was supported by grants from the National Science Foundation (BES-0222691, BES-0000303, and BES-0420840).


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Henry Lin
    • 1
  • Natalia M. Castro
    • 2
  • George N. Bennett
    • 3
  • Ka-Yiu San
    • 1
    • 4
  1. 1.Department of BioengineeringRice UniversityHoustonUSA
  2. 2.Department of ChemistryRice UniversityHoustonUSA
  3. 3.Department of Biochemistry and Cell BiologyRice UniversityHoustonUSA
  4. 4.Department of Chemical EngineeringRice UniversityHoustonUSA

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