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The effect of lactic acid on anaerobic carbon or nitrogen limited chemostat cultures of Saccharomyces cerevisiae

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Weak organic acids are well-known metabolic effectors in yeast and other micro-organisms. High concentrations of lactic acid due to infection of lactic acid bacteria often occurs in combination with growth under nutrient-limiting conditions in industrial yeast fermentations. The effects of lactic acid on growth and product formation of Saccharomyces cerevisiae were studied, with cells growing under carbon- or nitrogen-limiting conditions in anaerobic chemostat cultures (D=0.1 h−1) at pH values 3.25 and 5. It was shown that lactic acid in industrially relevant concentrations had a rather limited effect on the metabolism of S. cerevisiae. However, there was an effect on the energetic status of the cells, i.e. lactic acid addition provoked a reduction in the adenosine triphosphate (ATP) content of the cells. The decrease in ATP was not accompanied by a significant increase in the adenosine monophosphate levels.

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This work was financially supported by the Swedish Research Council (dnr 285-2000-656).

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Correspondence to Elisabeth Thomsson or Christer Larsson.

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Thomsson, E., Larsson, C. The effect of lactic acid on anaerobic carbon or nitrogen limited chemostat cultures of Saccharomyces cerevisiae . Appl Microbiol Biotechnol 71, 533–542 (2006). https://doi.org/10.1007/s00253-005-0195-3

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  • Lactic Acid
  • Benzoic Acid
  • Nitrogen Limitation
  • Carbon Limitation
  • Lactic Acid Concentration