Applied Microbiology and Biotechnology

, Volume 71, Issue 2, pp 234–237 | Cite as

Extraction of chitin from red crab shell waste by cofermentation with Lactobacillus paracasei subsp. tolerans KCTC-3074 and Serratia marcescens FS-3

  • W. J. Jung
  • G. H. Jo
  • J. H. Kuk
  • K. Y. Kim
  • R. D. ParkEmail author
Applied Microbial and Cell Physiology


For one-step extraction of chitin from red crab shell waste, cofermentation with Lactobacillus paracasei subsp. tolerans KCTC-3074, a lactic-acid-producing bacterium, and Serratia marcescens FS-3, a protease-producing bacterium, was conducted. Fermentation with single strain (L. 3074 or FS-3) was also conducted. At day 7, the pH in L. 3074, FS-3, and L. 3074+FS-3 (1:1) treatment decreased from 6.90 to 3.30, 5.88, and 3.48, respectively. Ash content in the residue after fermentation treatment of crab shells in L. 3074 and L. 3074+FS-3 (1:1) treatment drastically decreased from 41.2% to 3.19 and 1.15%, respectively. In L. 3074+FS-3 (1:1) cofermentation, the level of demineralization was the highest value of 97.2%, but the level of deproteinization in the cofermentation was 52.6% at day 7. Protein content in the treatment of FS-3 alone reduced from 22.4 to 3.62%. These results indicate that cofermentation of the shells using the two strains is efficient and applicable for the one-step extraction of crude chitin from red crab shell waste.


Fermentation Serratia Marcescens Crab Shell Shrimp Waste Total Titratable Acidity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by National Research Laboratory (NRL) program from the Ministry of Science and Technology (MOST), Korea.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • W. J. Jung
    • 1
  • G. H. Jo
    • 1
  • J. H. Kuk
    • 1
  • K. Y. Kim
    • 1
  • R. D. Park
    • 1
    Email author
  1. 1.Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and TechnologyChonnam National UniversityGwangjuSouth Korea

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